Medical and Biomedical Researches

Chiral Transformation of Cyanine Dye Aggregates Induced by Small Peptides.

June 29th, 2008
Chiral Transformation of Cyanine Dye Aggregates Induced by Small Peptides. Three small peptides (K4, K5, and K6) with different length were designed to induce the transformation of the assembled state and the chirality of cyanine dye supramolecule. The absorption and circular dichroism (CD) results indicated that, ...

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Thermally Changing Lattice Distance of Lamella in the Hydrated Solid of Octadecyltrimethylammonium C

June 29th, 2008
Thermally Changing Lattice Distance of Lamella in the Hydrated Solid of Octadecyltrimethylammonium Chloride. A temperature scanning small-angle X-ray scattering measurement was carried out for the hydrated solids of octadecyltrimethylammonium chloride (OTAC). A gradual change of the lattice spacing of lamella-like structure from 40 nm at 5 ...

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Characterization of Langmuir-Blodgett Film Using Differential Charging in X-ray Photoelectron Spectr

June 29th, 2008
Characterization of Langmuir-Blodgett Film Using Differential Charging in X-ray Photoelectron Spectroscopy. Differential charging is often regarded as a problem in X-ray photoelectron spectroscopy (XPS) studies, especially for insulating or partially conducting samples. Neutralization techniques have been developed to circumvent this effect. Instead of neutralizing the positive ...

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Interaction Between 14mer DNA Oligonucleotide and Cationic Surfactants of Various Chain Lengths.

June 29th, 2008
Interaction Between 14mer DNA Oligonucleotide and Cationic Surfactants of Various Chain Lengths. In the recent genomic era, a novel gene silencing approach has been introduced based on the use of small synthetic oligonucleotides, such as antisense RNAs, siRNAs, to inhibit the expression of a specific target ...

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Anomalous Diffusion of Fatty Acid Vesicles Driven by Adhesion Gradients.

June 29th, 2008
Anomalous Diffusion of Fatty Acid Vesicles Driven by Adhesion Gradients. The motion of fatty acid vesicles driven by adhesion gradients of a liquid substrate (Langmuir monolayer) has been investigated. Trajectories of the vesicle motion reveal not only heterogeneity among vesicles in different regions of the monolayer ...

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Surface-Directed Phase Separation via a Two-Step Quench Process in Binary Polymer Mixture Films with

June 29th, 2008
Surface-Directed Phase Separation via a Two-Step Quench Process in Binary Polymer Mixture Films with Asymmetry Compositions. Surface-directed phase separation via a two-step quench process in asymmetry polymer mixtures is numerically investigated by coupling the Flory-Huggins-de Gennes equation with the Cahn-Hilliard-Cook equation. Two distinct situations, i.e., the ...

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Protonation and Conformational Dynamics of GFP Mutants by Two-Photon Excitation Fluorescence Correla

June 29th, 2008
Protonation and Conformational Dynamics of GFP Mutants by Two-Photon Excitation Fluorescence Correlation Spectroscopy. GFP mutants are known to display fluorescence flickering, a process that occurs in a wide time range. Because serine 65, threonine 203, glutamate 222, and histidine 148 have been indicated as key residues ...

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Analysis of the Unfolding Process of Green Fluorescent Protein by Molecular Dynamics Simulation.

June 29th, 2008
Analysis of the Unfolding Process of Green Fluorescent Protein by Molecular Dynamics Simulation. Molecular dynamics simulation of the enforced stretching of circularly permuted green fluorescent protein (cpGFP) was performed to observe the detailed process of unfolding of beta-sheets in cpGFP and to clarify the structural change ...

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Molecular Dynamics Simulation Study of the Influence of Chirality on the Stability of the Smectic Q

June 29th, 2008
Molecular Dynamics Simulation Study of the Influence of Chirality on the Stability of the Smectic Q Liquid Crystal Phase. The structure of smectic Q (SmQ) liquid crystal phase consisting of a dichiral molecule, called M7BBM7, was studied by submicrosecond molecular dynamics (MD) simulation. A detailed atomic ...

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Molecular Dynamics Study of the Solvation of an alpha-Helical Transmembrane Peptide by DMSO.

June 29th, 2008
Molecular Dynamics Study of the Solvation of an alpha-Helical Transmembrane Peptide by DMSO. A 10-ns molecular dynamics study of the solvation of a hydrophobic transmembrane helical peptide in dimethyl sulfoxide (DMSO) is presented. The objective is to analyze how this aprotic polar solvent is able to ...

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Functional Clustering of Metastasis Proteins Describes Plastic Adaptation Resources of Breast-Cancer

June 29th, 2008
Functional Clustering of Metastasis Proteins Describes Plastic Adaptation Resources of Breast-Cancer Cells to New Microenvironments. To examine the molecular mechanisms underlying breast cancer metastasis in liver and search for potential markers of metastatic progression in soft-tissue, we analyzed metastatic variants developed from the highly metastatic MDA-MB ...

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Construction of a Proteome Profile and Functional Analysis of the Proteins Involved in the Initiatio

June 29th, 2008
Construction of a Proteome Profile and Functional Analysis of the Proteins Involved in the Initiation of Mouse Spermatogenesis. Spermatogenesis is a complex process of terminal differentiation wherein mature sperm are produced. In the first wave of mouse spermatogenesis, different spermatogenic cells appear at specific time points, ...

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A Proteomics Approach To Quantify Protein Levels Following RNA Interference: Case Study with Glutath

June 29th, 2008
A Proteomics Approach To Quantify Protein Levels Following RNA Interference: Case Study with Glutathione Transferase Superfamily from the Model Metazoan Caenorhabditis elegans. Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression ...

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Mitosis-Dependent Protein Expression in Neuroblastoma Cell Line N1E-115.

June 29th, 2008
Mitosis-Dependent Protein Expression in Neuroblastoma Cell Line N1E-115. Systematic work on differential protein expression in mitosis is limited, and we therefore used neuroblastoma cells (N1E-115) incubated with either colcemid or nocodazole to arrest mitosis. Proteins were identified by MALDI-TOF/TOF and nano-LC-ESI-MS/MS with subsequent quantification of spot ...

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Roaming Atoms and Radicals: A New Mechanism in Molecular Dissociation.

June 29th, 2008
Roaming Atoms and Radicals: A New Mechanism in Molecular Dissociation. The detailed description of chemical reaction rates is embodied in transition state theory (TST), now recognized as one of the great achievements of theoretical chemistry. TST employs a series of simplifying assumptions about the dynamical behavior ...

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Mass Spectrometry of Acoustically Levitated Droplets.

June 29th, 2008
Mass Spectrometry of Acoustically Levitated Droplets. Containerless sample handling techniques such as acoustic levitation offer potential advantages for mass spectrometry, by eliminating surfaces where undesired adsorption/desorption processes can occur. In addition, they provide a unique opportunity to study fundamental aspects of the ionization process as well ...

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Dynamically Multiplexed Ion Mobility Time-of-Flight Mass Spectrometry.

June 29th, 2008
Dynamically Multiplexed Ion Mobility Time-of-Flight Mass Spectrometry. Ion mobility spectrometry-time-of-flight mass spectrometry (IMS-TOFMS) has been increasingly used in analysis of complex biological samples. A major challenge is to transform IMS-TOFMS to a high-sensitivity, high-throughput platform, for example, for proteomics applications. In this work, we have developed ...

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Validation of an Extraction Method on Whole Pericarp of Grape Berry (Vitis vinifera L. cv. Shiraz) t

June 29th, 2008
Validation of an Extraction Method on Whole Pericarp of Grape Berry (Vitis vinifera L. cv. Shiraz) to Study Biochemical and Molecular Aspects of Flavan-3-ol Synthesis during Berry Development. An extraction method on grape berry was optimized for the total flavan-3-ol content measurement with regard to the ...

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Characterization of the Key Aroma Compounds in an American Bourbon Whisky by Quantitative Measuremen

June 29th, 2008
Characterization of the Key Aroma Compounds in an American Bourbon Whisky by Quantitative Measurements, Aroma Recombination, and Omission Studies. Thirty-one of the 45 odor-active compounds previously identified by us in an American Bourbon whisky were quantified by stable isotope dilution assays. Also for this purpose, new ...

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Effects of Thermal Treatments and Storage on Pectin Methylesterase and Peroxidase Activity in Freshl

June 29th, 2008
Effects of Thermal Treatments and Storage on Pectin Methylesterase and Peroxidase Activity in Freshly Squeezed Orange Juice. A specific indicator of freshness, allowing routine distinction between freshly squeezed orange juices (FSOJs) and FSOJ-like products, was to be identified. Using the Actijoule unit of a tubular heater ...

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Characterization of the High Molecular Weight Cd-Binding Complex in Water Hyacinth (Eichhornia crass

June 29th, 2008
Characterization of the High Molecular Weight Cd-Binding Complex in Water Hyacinth (Eichhornia crassipes) When Exposed to Cd. Water hyacinth ( Eichhornia crassipes) is a rapid-growing freshwater vascular plant that has been used to remove heavy metals in contaminated water. But the transportation and distribution of the ...

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Effect of Decontamination Agents on the Microbial Population, Sensorial Quality, and Nutrient Conten

June 29th, 2008
Effect of Decontamination Agents on the Microbial Population, Sensorial Quality, and Nutrient Content of Grated Carrots (Daucus carota L.). Several decontamination agents including water, sodium hypochlorite, peroxyacetic acid, neutral electrolyzed oxidizing water, and chlorine dioxide gas were tested for their effectiveness to reduce the natural microflora ...

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Isolation and Characterization of a New Hydroxytyrosol Derivative from Olive (Olea europaea) Leaves.

June 29th, 2008
Isolation and Characterization of a New Hydroxytyrosol Derivative from Olive (Olea europaea) Leaves. A new secoiridoid compound was isolated from the leaves of Olea europaea. This compound, not previously identified, is the bis methylacetal of oleuropein aglycone, the 3,4-dihydroxyphenylethyl [(2,6-dimethoxy-3-ethylidene)-tetrahydropyran-4-yl]acetate (3,4-DHPEA-DETA), and was found in different ...

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Role of Lysine epsilon-Amino Groups of beta-Lactoglobulin on Its Activating Effect of Kluyveromyces

June 29th, 2008
Role of Lysine epsilon-Amino Groups of beta-Lactoglobulin on Its Activating Effect of Kluyveromyces lactis beta-Galactosidase. Native beta-lactoglobulin binds and increases the activity of Kluyveromyces lactis beta-galactosidase. Construction of a three-dimensional (3D) model of beta-lactoglobulin showed that lysine residues 15, 47, 69, and 138 are the most ...

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Physical and Oxidative Stability of Fish Oil-in-Water Emulsions Stabilized with beta-Lactoglobulin a

June 29th, 2008
Physical and Oxidative Stability of Fish Oil-in-Water Emulsions Stabilized with beta-Lactoglobulin and Pectin. The oxidation of fatty acids can be inhibited by engineering the surface of oil-in-water emulsion droplets to decrease interactions between aqueous phase prooxidants and lipids. The objective of this research was to evaluate ...

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