Medical and Biomedical Researches

Evidence and the industrialization of medicine.

June 29th, 2008
Evidence and the industrialization of medicine. Medicine is changing rapidly. In part, this is due to the accumulation of discoveries in biomedical science. However, this is not sufficient to explain the changes clinicians see. Whereas once medical advance concerned discoveries external to clinical practice (such as ...

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Food contact hypersensitivity syndrome: the mucosal contact urticaria paradigm.

June 29th, 2008
Food contact hypersensitivity syndrome: the mucosal contact urticaria paradigm. Oral allergy syndrome (OAS) or pollen-fruit allergy syndrome represents a mucosal allergic contact urticaria in people sensitized to common pollens, due to IgE cross-reactivity between homologous pollen allergens and various plant foods. It is the most prevalent ...

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The role of physiotherapy after total knee arthroplasty in patients with haemophilia.

June 29th, 2008
The role of physiotherapy after total knee arthroplasty in patients with haemophilia. Summary. With the availability of clotting factor concentrates, advances in surgical techniques, better implant design, and improvements in postoperative management, total knee arthroplasty has become the treatment of choice for haemophilia patients suffering from ...

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G-protein-coupled receptors and melanoma.

June 29th, 2008
G-protein-coupled receptors and melanoma. G-protein-coupled receptors (GPCR) are the largest family of receptors with over 500 members. Evaluation of GPCR gene expression in primary human tumors identified over-expression of GPCR in several tumor types. Analysis of cancer samples in different disease stages also suggests that some ...

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Generation and Characterization of Monoclonal Antibodies to TDRD7 Protein.

June 29th, 2008
Generation and Characterization of Monoclonal Antibodies to TDRD7 Protein. Abstract TDRD7 is a scaffold protein whose specific function is unknown. It has been identified in complexes with proteins that regulate cytoskeleton dynamics and centrosomal movements, mRNA transport, and protein translation apparatus. Here we report the generation ...

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Preparation and characterization of monoclonal antibody against melatonin.

June 29th, 2008
Preparation and characterization of monoclonal antibody against melatonin. Abstract Anti-melatonin monoclonal antibodies (MAb) were prepared following coupling melatonin to bovine serum albumin (BSA) by Mannich reaction. Balb/c mice were immunized via injection of the melatonin-BSA intraperitonally. The spleen cells producing high titer of antibody were fused ...

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Generation and Characterization of Monoclonal Antibodies Against Klebsiella pneumoniae.

June 29th, 2008
Generation and Characterization of Monoclonal Antibodies Against Klebsiella pneumoniae. Abstract The present work was undertaken with the aim to generate highly specific monoclonal antibodies (MAb) targeted against Klebsiella pneumoniae that could be incorporated into a highly sensitive and simple ELISA (plate and dot) based system for ...

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Production and characterization of monoclonal antibody specific to recombinant dengue multi-epitope

June 29th, 2008
Production and characterization of monoclonal antibody specific to recombinant dengue multi-epitope protein. Abstract Monoclonal antibodies against novel dengue recombinant protein were produced following immunization of Balb/c mice with recombinant dengue multi-epitope protein (r-DMEP) expressed in Escherichia coli vector and purified in a single-step chromatography system. Antigenicity ...

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Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human NOB1 Protein.

June 29th, 2008
Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human NOB1 Protein. Abstract The human NOB1 gene is located at chromosome 16q22.1, encoding a protein consisting of one zinc ribbon domain. Here we aimed to efficiently generate a monoclonal antibody against NOB1 protein. We synthesized ...

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Production and characterization of monoclonal antibody specific for NS3 helicase of hepatitis C viru

June 29th, 2008
Production and characterization of monoclonal antibody specific for NS3 helicase of hepatitis C virus. Abstract Hepatitis C virus (HCV) infection is the major etiological agent of chronic hepatitis, which leads to liver cirrhosis and hepatocellular carcinomas. HCV NS3 helicase is a promising target of anti-virus therapy. ...

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Generation of Rat Monoclonal Antibodies Against Human RANTES.

June 29th, 2008
Generation of Rat Monoclonal Antibodies Against Human RANTES. Abstract RANTES (or regulated upon activation, normal T cell expressed and secreted) belongs to the rapidly growing chemokine family. It is mainly produced by T cells, epithelial cells, monocytes, fibroblasts, and mesanglial cells. Increased RANTES expression has been ...

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Generation and characterization of a single-chain fv antibody against gli3, a hedgehog signaling pat

June 29th, 2008
Generation and characterization of a single-chain fv antibody against gli3, a hedgehog signaling pathway transcription factor. Abstract Gli3 is a key regulator of development, controlling multiple patterning steps. Here we report the generation of a scFv antibody specific to the repressor domain of human Gli3. We ...

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Generation and Characterization of Monoclonal Antibodies to NIAM: A Nuclear Interactor of ARF and Md

June 29th, 2008
Generation and Characterization of Monoclonal Antibodies to NIAM: A Nuclear Interactor of ARF and Mdm2. Abstract Nuclear interactor of ARF and Mdm2 (NIAM) is a newly discovered growth inhibitor that helps maintain chromosomal stability. It is functionally linked to the ARF-Mdm2-p53 tumor suppressor pathway and is ...

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Mouse Monoclonal Immunoglobulin E Antibodies Specific for the Microsporidian Encephalitozoon cunicul

June 29th, 2008
Mouse Monoclonal Immunoglobulin E Antibodies Specific for the Microsporidian Encephalitozoon cuniculi Polar Tube Protein 1. Abstract The microsporidian Encephalitozoon cuniculi is a spore-forming, obligate, intracellular parasitic pathogen with a unique organelle called a polar tube, the extrusion of which is essential for invading a host cell. ...

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Generation of Functional Blocking Monoclonal Antibodies Against Mouse Interleukin-12 p40 Homodimer a

June 29th, 2008
Generation of Functional Blocking Monoclonal Antibodies Against Mouse Interleukin-12 p40 Homodimer and Monomer. Abstract The role of interleukin (IL)-12 (p40:p35) and IL-23 (p40:p19) is becoming clear in immune response and inflammation. However, biological functions of IL-12 p40 homodimer (p40(2)) and monomer (p40) remain poorly understood due ...

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Long-Term Prognosis of HIV-Infected Patients with Kaposi Sarcoma Treated with Pegylated Liposomal Do

June 29th, 2008
Long-Term Prognosis of HIV-Infected Patients with Kaposi Sarcoma Treated with Pegylated Liposomal Doxorubicin. Introduction. @nbsp; Incidence of Kaposi sarcoma (KS) in human immunodeficiency virus (HIV)-infected persons has dramatically decreased in the highly active antiretroviral therapy era. However, this tumor still represents the most common cancer in ...

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Comparison of the Clinical Effectiveness of Oseltamivir and Zanamivir against Influenza Virus Infect

June 29th, 2008
Comparison of the Clinical Effectiveness of Oseltamivir and Zanamivir against Influenza Virus Infection in Children. Background. @nbsp; We compared the clinical effectiveness of oseltamivir and zanamivir in children with influenza A (H1N1) virus, influenza A (H3N2) virus, and influenza B virus infections. Methods. @nbsp; Total febrile ...

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The Management of Encephalitis: Clinical Practice Guidelines by the Infectious Diseases Society of A

June 29th, 2008
The Management of Encephalitis: Clinical Practice Guidelines by the Infectious Diseases Society of America. Guidelines for the diagnosis and treatment of patients with encephalitis were prepared by an Expert Panel of the Infectious Diseases Society of America. The guidelines are intended for use by health care ...

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Pediatric Human Immunodeficiency Virus Infection and Circulating IgD(+) Memory B Cells.

June 29th, 2008
Pediatric Human Immunodeficiency Virus Infection and Circulating IgD(+) Memory B Cells. Levels of circulating naive and memory B cells were measured in human immunodeficiency virus (HIV)-infected children and control subjects to determine whether the irreversible depletion of memory B cells described in HIV-infected adults occurs in ...

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Host- and Microbe-Related Risk Factors for and Pathophysiology of Fatal Rickettsia conorii Infection

June 29th, 2008
Host- and Microbe-Related Risk Factors for and Pathophysiology of Fatal Rickettsia conorii Infection in Portuguese Patients. Background. @nbsp; The pathophysiologic mechanisms that determine the severity of Mediterranean spotted fever (MSF) and the host-related and microbe-related risk factors for a fatal outcome are incompletely understood. Methods. @nbsp; ...

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Nevirapine Resistance in Women and Infants after First versus Repeated Use of Single-Dose Nevirapine

June 29th, 2008
Nevirapine Resistance in Women and Infants after First versus Repeated Use of Single-Dose Nevirapine for Prevention of HIV-1 Vertical Transmission. Single-dose (SD) nevirapine (NVP) significantly reduces mother-to-child transmission of human immunodeficiency virus (HIV). We analyzed NVP resistance after receipt of SD NVP in 57 previously SD ...

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Regulatory Role of Interleukin-10 and Interferon-gamma in Severe Hepatic Central and Peripheral Fibr

June 29th, 2008
Regulatory Role of Interleukin-10 and Interferon-gamma in Severe Hepatic Central and Peripheral Fibrosis in Humans Infected with Schistosoma japonicum. Background. @nbsp; Schistosoma japonicum is the most pathogenic agent of hepatosplenic schistosomiasis. It causes fibrosis of the central (CentF) and peripheral (PerF) portal areas. We investigated whether ...

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Is an Increased Dwell Time of a Peripherally Inserted Catheter Associated With an Increased Risk of

June 29th, 2008
Is an Increased Dwell Time of a Peripherally Inserted Catheter Associated With an Increased Risk of Bloodstream Infection in Infants? Objective. @nbsp; To estimate the risk of bloodstream infection associated with catheter dwell time in infants. Design. @nbsp; Retrospective study. Setting. @nbsp; Duke University Medical Center ...

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Safety and Immunogenicity of a New Tuberculosis Vaccine, MVA85A, in Healthy Adults in South Africa.

June 29th, 2008
Safety and Immunogenicity of a New Tuberculosis Vaccine, MVA85A, in Healthy Adults in South Africa. Background. @nbsp; The efficacy of bacille Calmette-Guérin (BCG) may be enhanced by heterologous vaccination strategies that boost the BCG-primed immune response. One leading booster vaccine, MVA85A (where \"MVA\" denotes \"modified vaccinia ...

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alpha(+)-Thalassemia Protects against Anemia Associated with Asymptomatic Malaria: Evidence from Com

June 29th, 2008
alpha(+)-Thalassemia Protects against Anemia Associated with Asymptomatic Malaria: Evidence from Community-Based Surveys in Tanzania and Kenya. Background. @nbsp; In hospital-based studies, alpha(+)-thalassemia has been found to protect against severe, life-threatening falciparum malaria. alpha(+)-Thalassemia does not seem to prevent infection or high parasite densities but rather limits ...

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