Medical and Biomedical Researches

Differential expression of GADD45beta in normal and osteoarthritic cartilage: Potential role in home

June 27th, 2008
Differential expression of GADD45beta in normal and osteoarthritic cartilage: Potential role in homeostasis of articular chondrocytes. OBJECTIVE: Our previous study suggested that growth arrest and DNA damage-inducible protein 45beta (GADD45beta) prolonged the survival of hypertrophic chondrocytes in the developing mouse embryo. This study was undertaken, therefore, ...

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Modeling and analysis of diffusion and reaction in legume nodules.

June 27th, 2008
Modeling and analysis of diffusion and reaction in legume nodules. Diffusion of gases through legume nodules is important for nitrogen fixation. A mathematical model is presented for diffusion and enzymatic reaction for legume nodules with a reactive core and an inert shell. The transient model is ...

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Modeling bisubstrate removal by biofilms.

June 27th, 2008
Modeling bisubstrate removal by biofilms. A bisubstrate secondary utilization model is based on the concept that an individual substrate can be utilized not only by the biomass by its utilization but also by the biomass made from the utilization of the other substrate. When substrate concentrations ...

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Performance of the duckweed species Lemna gibba on municipal wastewater for effluent renovation and

June 27th, 2008
Performance of the duckweed species Lemna gibba on municipal wastewater for effluent renovation and protein production. An outdoor experiment was conducted in miniponds to evaluate the performance of Lemna gibba, a duckweed species, as a domestic wastewater stripper. Duckweed is one of the floating plants with ...

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Oxidation of hydrogen sulfide by Thiobacillus denitrificans: Desulfurization of natural gas.

June 27th, 2008
Oxidation of hydrogen sulfide by Thiobacillus denitrificans: Desulfurization of natural gas. It has been demonstrated that Thiobacillus denitrificans may be readily cultivated anaerobically in batch reactors on H(2)S (g) under sulfide-limiting conditions. Under these conditions sulfide concentrations in the culture medium were less than 1 muM, ...

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Generalization of monod kinetics for analysis of growth data with substrate inhibition.

June 27th, 2008
Generalization of monod kinetics for analysis of growth data with substrate inhibition. The inhibitory effect of butanol on yeast growth has been studied for the strain Candida utilis ATCC 8205 growing aerobically on butanol under batch conditions. A mathematical expression was then proposed to fit the ...

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Immobilization of cellulolytic and hemicellulolytic enzymes on inorganic supports.

June 27th, 2008
Immobilization of cellulolytic and hemicellulolytic enzymes on inorganic supports. Commercial cellulase preparations from Trichoderma viride and Aspergillus niger were immobilized on porous silica glass and ceramics such as alumina and titania with titanium tetrachloride (TiCl(4)) and on their silanized derivatives with glutaraldehyde (GLUT). The amounts of ...

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Steam pretreatment of lignocellulosic material for enhanced enzymatic hydrolysis.

June 27th, 2008
Steam pretreatment of lignocellulosic material for enhanced enzymatic hydrolysis. Pretreatment methods were compared with steam explosion, and differing views on the relative importance of mechanical and chemical effects were outlined. Hydrolysis was desirable; pyrolysis was undesirable. The effects of initial moisture content on steam consumption, mechanism ...

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Vortex chamber for in situ recovery of the antibiotic myxovirescin A in continuous cultivation.

June 27th, 2008
Vortex chamber for in situ recovery of the antibiotic myxovirescin A in continuous cultivation. A vortex chamber for continuous adsorption of the antibiotic Myxovirescin A on XAD resins was developed. In this paper the design and use of the vortex chamber in an external bypass of ...

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Effect of growth rate and expression level on plasmid stability in Saccharomyces cerevisiae.

June 27th, 2008
Effect of growth rate and expression level on plasmid stability in Saccharomyces cerevisiae. An auxotrophic mutant of Saccharomyces cerevisiae, containing a recombinant 2-mu-based plasmid, was grown in selective media in continuous culture. The plasmid retained the ability to synthesize acid phosphatase as product, which was deleted ...

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Chronocoulometric techniques in the study of computerized enzyme electrode systems.

June 27th, 2008
Chronocoulometric techniques in the study of computerized enzyme electrode systems. The electrochemical transient of a two-substrate enzyme electrode was studied theoretically and experimentally. Operation of such electrodes in the chronocoulometric mode leads to increased electrode sensitivity and makes possible the retrieval of useful information on transport ...

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Modeling and static optimization of the ethanol production in a cascade reactor. II. Static optimiza

June 27th, 2008
Modeling and static optimization of the ethanol production in a cascade reactor. II. Static optimization. In Part I(1) of this research a complex model was obtained for describing the ethanol fermentation in a cascade reactor. This complexity is due to both the nonlinearity and the large ...

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Modeling and static optimization of the ethanol production in a cascade reactor. I. Modeling.

June 27th, 2008
Modeling and static optimization of the ethanol production in a cascade reactor. I. Modeling. Let us consider the modeling of a cascade reactor for the production of ethanol. The rates of reaction in alcoholic fermentation are modeled so that it can assume both ethanol and substrate ...

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Scaling oxygen mass transfer in agitated fermentors.

June 27th, 2008
Scaling oxygen mass transfer in agitated fermentors. There are many scaling formulas that predict the oxygen mass transfer coefficient as k(L).a = constant.(Hp/V)(alpha)Vs(beta) Exponents alpha and beta frequently are scale dependent themselves. A general formula has been derived from the work of Calderbank,(1) Miller,(2) and Tilton,(3) ...

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Fractionation of Aspergillus niger cellulases by combined ion exchange affinity chromatography.

June 27th, 2008
Fractionation of Aspergillus niger cellulases by combined ion exchange affinity chromatography. Eight chemically modified cellulose supports were tested for their ability to absorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to ...

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Nature of yeast cells immobilized by radiation polymerization. Activity dependence on the water cont

June 27th, 2008
Nature of yeast cells immobilized by radiation polymerization. Activity dependence on the water content of polymer carriers. Higher activity of ethanol production due to higher density of yeast cells was observed in yeast cells immobilized with artificial polymer carriers of higher water content based on methoxypolyethyleneglycol ...

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Pretreatment of lignocellulosic materials with hydrogen peroxide in presence of manganese compounds.

June 27th, 2008
Pretreatment of lignocellulosic materials with hydrogen peroxide in presence of manganese compounds. Pretreatment of lignocellulosic materials such as newspaper, rice straw, pulp waste, and municipal solid waste with hydrogen peroxide in the presence of manganese compounds greatly enhances their susceptibility to enzymatic saccharification. This pretreatment can ...

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Cellulase immobilized on poly-L-glutamic acid.

June 27th, 2008
Cellulase immobilized on poly-L-glutamic acid. Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be ...

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Biodehalogenation of bromotrichloromethane and 1,2-dibromo-3-chloropropane by Pseudomonas putida PpG

June 27th, 2008
Biodehalogenation of bromotrichloromethane and 1,2-dibromo-3-chloropropane by Pseudomonas putida PpG-786. Biodehalogenation of 10(-5) M concentrations of bromotrichloromethane (BTM) and 1,2-dibromo-3-chloropropane (DBCP) was studied in static cultures of Pseudomonas putida PpG-786. The experimental cultures were prepared by growing P. putida on camphor, which is known to induce the ...

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Current literature in mass spectrometry.

June 27th, 2008
Current literature in mass spectrometry. In order to keep subscribers up-to-date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. ...

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Rapid characterization of fatty alcohol ethoxylates by non-aqueous capillary electrophoresis.

June 27th, 2008
Rapid characterization of fatty alcohol ethoxylates by non-aqueous capillary electrophoresis. Fatty alcohol ethoxylates (FAE) (a mixture of nonionic surfactants) have been characterized using NACE with UV detection. Phenyl polyurethane derivatives of these compounds were previously obtained by reaction with phenyl isocyanate. The derivatization reaction only requires ...

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Carbon nanotube disposable detectors in microchip capillary electrophoresis for water-soluble vitami

June 27th, 2008
Carbon nanotube disposable detectors in microchip capillary electrophoresis for water-soluble vitamin determination: Analytical possibilities in pharmaceutical quality control. In this work, the synergy of one mature example from \"lab-on-chip\" domain, such as CE microchips with emerging miniaturized carbon nanotube detectors in analytical science, is presented. Two ...

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Single-cell gel electrophoresis (the comet assay): Loops or fragments?

June 27th, 2008
Single-cell gel electrophoresis (the comet assay): Loops or fragments? Single-cell gel electrophoresis, or the comet assay, is widely used to measure DNA damage and repair. Upon electrophoresis, the DNA of lysed, agarose-embedded cells known as nucleoids, extends towards the anode in a structure resembling a comet, ...

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High-sensitivity capillary electrophoresis for speciation of organomercury in biological samples usi

June 27th, 2008
High-sensitivity capillary electrophoresis for speciation of organomercury in biological samples using hollow fiber-based liquid-liquid-liquid microextraction combined with on-line preconcentration by large-volume sample stacking. A hollow fiber-based liquid-liquid-liquid microextraction (HF-LLLME) combined with on-line large-volume sample stacking (LVSS) has been developed for the speciation of organomercury in biological ...

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Influence of polyelectrolyte coating conditions on capillary coating stability and separation effici

June 27th, 2008
Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis. Polyelectrolytes are widely used in capillary electrophoresis as coating agents of silica capillaries to prevent adsorption phenomena and improve the repeatability of peptide and protein analysis. A systematic study of the ...

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