Medical and Biomedical Researches

Comprehensive Royal Jelly (RJ) Proteomics Using One- and Two-Dimensional Proteomics Platforms Reveal

June 27th, 2008
Comprehensive Royal Jelly (RJ) Proteomics Using One- and Two-Dimensional Proteomics Platforms Reveals Novel RJ Proteins and Potential Phospho/Glycoproteins. Royal jelly (RJ) is an exclusive food for queen honey bee ( Apis mellifera L.) that is synthesized and secreted by young worker bees. RJ is also widely ...

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Amazing Stability of Phosphate-Quaternary Amine Interactions.

June 27th, 2008
Amazing Stability of Phosphate-Quaternary Amine Interactions. We have previously used MALDI mass spectrometry to highlight ammonium- or guanidinium-aromatic interactions via cation-pi bonding and ammonium- or guanidinium-phosphate interactions through salt bridge formation. In the present work, the gas-phase stability and dissociation pathways of the interaction between phosphorylated ...

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Isocratic Solid Phase Extraction-Liquid Chromatography (SPE-LC) Interfaced to High-Performance Tande

June 27th, 2008
Isocratic Solid Phase Extraction-Liquid Chromatography (SPE-LC) Interfaced to High-Performance Tandem Mass Spectrometry for Rapid Protein Identification. Reversed-phase liquid chromatography interfaced to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) allows analysis of very complex peptide mixtures at great sensitivity, but it can be very time-consuming, typically using 60 ...

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Design, Synthesis, and Biological Evaluation of Novel Tri- and Tetrasubstituted Imidazoles as Highly

June 27th, 2008
Design, Synthesis, and Biological Evaluation of Novel Tri- and Tetrasubstituted Imidazoles as Highly Potent and Specific ATP-Mimetic Inhibitors of p38 MAP Kinase: Focus on Optimized Interactions with the Enzyme\'s Surface-Exposed Front Region. The synthesis, biological testing, and SAR of novel 2,4,5- and 1,2,4,5-substituted 2-thioimidazoles are described. ...

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Piperazine Sulfonamides as Potent, Selective, and Orally Available 11beta-Hydroxysteroid Dehydrogena

June 27th, 2008
Piperazine Sulfonamides as Potent, Selective, and Orally Available 11beta-Hydroxysteroid Dehydrogenase Type 1 Inhibitors with Efficacy in the Rat Cortisone-Induced Hyperinsulinemia Model. 11beta-Hydroxysteroid dehydrogenase type 1 (11beta-HSD1) is the enzyme that converts cortisone to cortisol. Evidence suggests that selective inhibition of 11beta-HSD1 could treat diabetes and metabolic ...

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Tripod-like Cationic Lipids as Novel Gene Carriers.

June 27th, 2008
Tripod-like Cationic Lipids as Novel Gene Carriers. An innovative family of tridentate-cationic \"single-chained lipids\" designed to enhance DNA compaction and to promote endosomal escape was synthesized by coupling various lipids to a multibranched scaffold. DNA retardation assays confirmed the ability of the most members of the ...

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Molecular Structures and Energetics of the (TiO2)n (n = 1-4) Clusters and Their Anions.

June 27th, 2008
Molecular Structures and Energetics of the (TiO2)n (n = 1-4) Clusters and Their Anions. The (TiO 2) n clusters and their anions for n = 1-4 have been studied with coupled cluster theory [CCSD(T)] and density functional theory (DFT). For n > 1, numerous conformations are ...

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beta-Peptidic Peptidomimetics.

June 27th, 2008
beta-Peptidic Peptidomimetics. For more than a decade now, a search for answers to the following two questions has taken us on a new and exciting journey into the world of beta- and gamma-peptides: What happens if the oxygen atoms in a 3 i -helix of a ...

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Kinesin Tail Domains and Mg(2+) Directly Inhibit Release of ADP from Head Domains in the Absence of

June 27th, 2008
Kinesin Tail Domains and Mg(2+) Directly Inhibit Release of ADP from Head Domains in the Absence of Microtubules. Kinesin-1 is a vesicle motor that can fold into a compact inhibited conformation that is produced by interaction of the heavy chain C-terminal tail region with the N-terminal ...

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Hydrogen Location in Stages of an Enzyme-Catalyzed Reaction: Time-of-Flight Neutron Structure of d-X

June 27th, 2008
Hydrogen Location in Stages of an Enzyme-Catalyzed Reaction: Time-of-Flight Neutron Structure of d-Xylose Isomerase with Bound d-Xylulose. The time-of-flight neutron Laue technique has been used to determine the location of hydrogen atoms in the enzyme d-xylose isomerase (XI). The neutron structure of crystalline XI with bound ...

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Mutations of Glucocorticoid Receptor Differentially Affect AF2 Domain Activity in a Steroid-Selectiv

June 27th, 2008
Mutations of Glucocorticoid Receptor Differentially Affect AF2 Domain Activity in a Steroid-Selective Manner To Alter the Potency and Efficacy of Gene Induction and Repression. The transcriptional activity of steroid hormones is intimately associated with their structure. Deacylcortivazol (DAC) contains several features that were predicted to make ...

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Clostridium acetobutylicum 8-Oxoguanine DNA Glycosylase (Ogg) Differs from Eukaryotic Oggs with Resp

June 27th, 2008
Clostridium acetobutylicum 8-Oxoguanine DNA Glycosylase (Ogg) Differs from Eukaryotic Oggs with Respect to Opposite Base Discrimination. During repair of damaged DNA, the oxidized base 8-oxoguanine (8-oxoG) is removed by 8-oxoguanine-DNA glycosylase (Ogg) in eukaryotes and most archaea, whereas in most bacteria it is removed by formamidopyrimidine-DNA ...

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(13)C Relaxation Studies of the DNA Target Sequence for HhaI Methyltransferase Reveal Unique Motiona

June 27th, 2008
(13)C Relaxation Studies of the DNA Target Sequence for HhaI Methyltransferase Reveal Unique Motional Properties. The goal of this work was to examine if sequence-dependent conformational flexibility in DNA plays a role in base extrusion, a common conformational change induced by many DNA-modifying enzymes. We studied ...

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Phosphorylation Analysis of G Protein-Coupled Receptor by Mass Spectrometry: Identification of a Pho

June 27th, 2008
Phosphorylation Analysis of G Protein-Coupled Receptor by Mass Spectrometry: Identification of a Phosphorylation Site in V2 Vasopressin Receptor. Phosphorylation plays vital roles in the regulation and function of the V2 vasopressin receptor (V2R), a G protein-coupled receptor (GPCR) that is responsible for maintaining water homeostasis in ...

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Localization and Quantitative Analysis of Antigen-Antibody Binding on 2D Substrate Using Imaging Nan

June 27th, 2008
Localization and Quantitative Analysis of Antigen-Antibody Binding on 2D Substrate Using Imaging NanoSIMS. Iron containing-antigen bound specifically to antibody immobilized on a surface is analyzed by nanoscale secondary ion mass spectrometry (NanoSIMS). This technique is well adapted compared with X-ray photoelectron spectroscopy and energy dispersive spectroscopy, ...

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Detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in Water U

June 27th, 2008
Detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in Water Using a Flow-Through Chemiluminescence Microarray Readout System. Fast, sensitive, and especially, multianalyte test systems are currently of high interest for the monitoring and quality control of drinking water, since traditional microbiological methods are labor ...

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Mass Spectrometry Analysis of Proteome-Wide Proteolytic Post-Translational Degradation of Proteins.

June 27th, 2008
Mass Spectrometry Analysis of Proteome-Wide Proteolytic Post-Translational Degradation of Proteins. Protein proteolytic degradation is an essential component to proper cell function and its life cycle. Here, we study the protein degradation in yeast Saccharomyces cerevisiae cells on a proteome-wide scale by detection of the intermediate peptides ...

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Digestion of Native Proteins for Proteomics Using a Thermocycler.

June 27th, 2008
Digestion of Native Proteins for Proteomics Using a Thermocycler. Efficient protein digestion is a critical step for successful mass spectrometry analysis. Here we describe simultaneous tryptic digestion and gradual unfolding of native proteins by application of a temperature gradient using a single cycle of 5 min ...

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Mechanistic Studies on the Catalytic Asymmetric Mannich-Type Reaction with Dihydroisoquinolines and

June 27th, 2008
Mechanistic Studies on the Catalytic Asymmetric Mannich-Type Reaction with Dihydroisoquinolines and Development of Oxidative Mannich-Type Reactions Starting from Tetrahydroisoquinolines. Detailed mechanistic studies on our recently reported asymmetric addition reactions of malonates to dihydroisoquinolines (DHIQs) catalyzed by chiral Pd(II) complexes were carried out. It was found that ...

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Reactivity of 13,13-Dibromo-2,4,9,11-tetraoxadispiro[5.0.5.1]tridecane toward Organolithiums: Remark

June 27th, 2008
Reactivity of 13,13-Dibromo-2,4,9,11-tetraoxadispiro[5.0.5.1]tridecane toward Organolithiums: Remarkable Resistance to the DMS Rearrangement. Reactions of dibromocyclopropane 2a, containing two spiro-fused 1,3-dioxane rings, with MeLi gave only the methylation products 8 and 9 even at elevated temperatures. In contrast, the cyclohexane analogue 2b treated with MeLi underwent a smooth ...

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Photosensitized Oxidation of Alkyl Phenyl Sulfoxides. C-S Bond Cleavage in Alkyl Phenyl Sulfoxide Ra

June 27th, 2008
Photosensitized Oxidation of Alkyl Phenyl Sulfoxides. C-S Bond Cleavage in Alkyl Phenyl Sulfoxide Radical Cations. The 3-cyano- N-methylquinolinium perchlorate (3-CN-NMQ (+) ClO 4 (-))-photosensitized oxidation of phenyl alkyl sulfoxides (PhSOCR 1R 2R 3, 1, R 1 = R 2 = H, R 3 = Ph; 2, ...

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C-H Carbene Insertion of alpha-Diazo Acetamides by Photolysis in Non-Conventional Media.

June 27th, 2008
C-H Carbene Insertion of alpha-Diazo Acetamides by Photolysis in Non-Conventional Media. Light from a mercury vapor high-pressure lamp was used to induce the photolytic decomposition of alpha-diazo acetamides in hexane and in nonconventional media such as water or a film. The corresponding beta- and/or gamma-lactams were ...

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Electrospun Fibrous Mats with High Porosity as Potential Scaffolds for Skin Tissue Engineering.

June 27th, 2008
Electrospun Fibrous Mats with High Porosity as Potential Scaffolds for Skin Tissue Engineering. Diffusional limitations of mass transport have adverse effects on engineering tissues that normally have high vascularity and cellularity. The current electrospinning method is not always successful to create micropores to encourage cell infiltration ...

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One-Pot Synthesis of Oligosaccharides by Combining Reductive Openings of Benzylidene Acetals and Gly

June 27th, 2008
One-Pot Synthesis of Oligosaccharides by Combining Reductive Openings of Benzylidene Acetals and Glycosylations. Combining triflic acid-promoted glycosylations of trichloroacetimidates with reductive opening of benzylidene acetals with triflic acid and triethylsilane as one-pot procedures provides access to a wide range of disaccharides and 2,4- and 3,4-branched trisaccharides. Vohra ...

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Phosphorous Acid and Urea: Valuable Sources of Phosphorus and Nitrogen in the Hydrothermal Synthesis

June 27th, 2008
Phosphorous Acid and Urea: Valuable Sources of Phosphorus and Nitrogen in the Hydrothermal Synthesis of Ammonium-Thorium Phosphates. Microcrystals of the first ammonium-thorium phosphates, (NH 4) 2Th(PO 4) 2.H 2O (tetragonal, I4 1/ amd, a = 7.0192(4) A, c = 17.9403(8) A) and NH 4Th 2(PO 4) ...

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