Medical and Biomedical Researches

[Determination of alliin and its related substances in garlic using pre-column derivatization and re

June 29th, 2008
[Determination of alliin and its related substances in garlic using pre-column derivatization and reversed-phase high performance liquid chromatography] A reversed-phase high performance liquid chromatographic method with pre-column derivatization for the determination of alliin and its related substances, which are the precursors of garlic\'s active components, was ...

Posted in researches 5 | No Comments »

[Separation of geometrical isomers of {Fe [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine]3} 2+ ([Fe(PDT)

June 29th, 2008
[Separation of geometrical isomers of {Fe [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine]3} 2+ ([Fe(PDT)3] 2+) using ion-pair reversed-phase high performance liquid chromatography] A method has been developed for the separation of two geometrical isomers of the {Fe[3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine]3}2+([Fe(PDT)3]2+) using ion-pair reversed-phase high performance liquid chromatography (RP-HPLC). The effects of the chromatographic conditions, ...

Posted in researches 5 | No Comments »

[Selection of back-ground electrolyte in capillary zone electrophoresis by triangle and tetrahedron

June 29th, 2008
[Selection of back-ground electrolyte in capillary zone electrophoresis by triangle and tetrahedron optimization methods] The triangle and tetrahedron optimization methods were developed for the selection of back-ground electrolyte (BGE) in capillary zone electrophoresis (CZE). Chromatographic fingerprint index F and chromatographic fingerprint relative index F(r) were used ...

Posted in researches 5 | No Comments »

[Determination of beta-adrenergic agonists in pig urine and pig fodder using miniaturized capillary

June 29th, 2008
[Determination of beta-adrenergic agonists in pig urine and pig fodder using miniaturized capillary electrophoresis with electrochemical detection] A method of miniaturized capillary electrophoresis with electrochemical detection has been developed for the determination of beta-adrenergic agonists in pig urine and pig fodder. Several important factors, including running ...

Posted in researches 5 | No Comments »

[Determination of tropane alkaloid components in Przewalskia tangutica Maxim. by capillary electroph

June 29th, 2008
[Determination of tropane alkaloid components in Przewalskia tangutica Maxim. by capillary electrophoresis with electrochemiluminescence detection] Based on an Eu-PB modified platinum electrode as the working electrode, a method for the simultaneous determination of the four tropane alkaloids, anisodamine, scopolamine, atropine and anisodine, by capillary electrophoresis with ...

Posted in researches 5 | No Comments »

[Extraction, preparation and identification of volatile compounds in Changyu XO brandy]

June 29th, 2008
[Extraction, preparation and identification of volatile compounds in Changyu XO brandy] A method for the preparation of volatile compounds in Changyu XO brandy was established. The volatile compounds were extracted using liquid-liquid extraction and then were separated into two fractions, namely, the acidic/water-soluble fraction and the ...

Posted in researches 5 | No Comments »

[Preparation and identification of recombinant human interferon-gamma]

June 29th, 2008
[Preparation and identification of recombinant human interferon-gamma] The renaturation with simultaneous purification of recombinant human interferon-gamma (rhIFN-gamma) expressed as inclusion bodies in Escherichia coli (E. coli) was accomplished by the stationary phase of hydrophobic interaction chromatography (STHIC) with the end group of poly(ethylene glycol) (PEG)(PEG200) packed ...

Posted in researches 5 | No Comments »

[Preparation of 1-(2-naphthyl) -3-methyl-5-pyrazolone as pre-column derivatization reagent for the d

June 29th, 2008
[Preparation of 1-(2-naphthyl) -3-methyl-5-pyrazolone as pre-column derivatization reagent for the determination of saccharides using high performance liquid chromatography-mass spectrometry] Eight saccharides were derivatized using 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) as pre-column derivatizing reagent, and separated on a reversed-phase Hypersil ODS 2 column (4.6 mm x 200 mm, 5 microm), ...

Posted in researches 5 | No Comments »

[Phosphopeptide enrichment strategy based on strong cation exchange chromatography]

June 29th, 2008
[Phosphopeptide enrichment strategy based on strong cation exchange chromatography] The routine analysis of phosphoproteomics needs effective and specific enrichment methods. The strong cation exchange chromatography (SCX) separation conditions, including the buffer solution and the separation gradient, were studied to enrich the phosphopeptides in a short time. ...

Posted in researches 5 | No Comments »

[Identification and relative quantification of peptides by capillary reversed-phase liquid chromatog

June 29th, 2008
[Identification and relative quantification of peptides by capillary reversed-phase liquid chromatography-tandem mass spectrometry] A method for the identification and relative quantification of peptides by capillary liquid chromatography ion-trap tandem mass spectrometry (LC-IT-MS/MS) was established. At first, the peptides were automatically identified by correlating the tandem mass ...

Posted in researches 5 | No Comments »

[Determination of three bufogenins in toad venom using reversed-phase high performance liquid chroma

June 29th, 2008
[Determination of three bufogenins in toad venom using reversed-phase high performance liquid chromatography] A reversed-phase high performance liquid chromatographic method for the quantitative determination of three bufogenins in toad venom was established. An Alltech Alltima C18 column (250 mm x 4.6 mm, 5 microm) was used. ...

Posted in researches 5 | No Comments »

[Development of full-quantified fingerprint for quality control of traditional Chinese medicines]

June 29th, 2008
[Development of full-quantified fingerprint for quality control of traditional Chinese medicines] Full-quantified fingerprint technique combines the fingerprint technique and multiple-target determination technique. The development of full-quantified fingerprint technique includes the preparation of full-quantified fraction, the quality control in preparation process with fingerprint technique and determination of ...

Posted in researches 5 | No Comments »

[Effect of fingerprintology of traditional Chinese medicine (TCM) in the innovative development of T

June 29th, 2008
[Effect of fingerprintology of traditional Chinese medicine (TCM) in the innovative development of TCM] The background and main task of the system of TCM fingerprintology from holism, systematology and complexity science are elaborately discussed. The fingerprintology of TCM is a novel system, in which the pharmacologic ...

Posted in researches 5 | No Comments »

[Chromatographic fingerprint and quality control of traditional Chinese medicines]

June 29th, 2008
[Chromatographic fingerprint and quality control of traditional Chinese medicines] The development of modern chromatographic technologies and related chemometric methods in the research of chromatographic fingerprint, and their applications in quality control of traditional Chinese medicines (TCMs) are comprehensively reviewed. Furthermore, we preliminarily discuss the quality control ...

Posted in researches 5 | No Comments »

[Quality control of traditional Chinese medicines by the capillary electrophoresis fingerprint and c

June 29th, 2008
[Quality control of traditional Chinese medicines by the capillary electrophoresis fingerprint and capillary electrophoresis-mass spectrometry] The quality control of traditional Chinese medicines by capillary electrophoresis (CE) fingerprint and CE-mass spectrometry(CE-MS) is reviewed. The optimum experimental conditions of CE, the research and appraisal methods of CE fingerprint ...

Posted in researches 5 | No Comments »

[Quality control of Chinese herbal medicines with chromatographic fingerprints]

June 29th, 2008
[Quality control of Chinese herbal medicines with chromatographic fingerprints] The applications of chromatographic fingerprint in the quality control of Chinese herbal medicines (CHMs), such as chemical fingerprint in the authentication of CHMs, chromatographic pharmacodynamics, biofingerprint and metabolic fingerprint in the efficacy evaluation of CHMs are reviewed. ...

Posted in researches 5 | No Comments »

[Some advances and prospects in fingerprintings of traditional Chinese medicines]

June 29th, 2008
[Some advances and prospects in fingerprintings of traditional Chinese medicines] The quality control standards are the key points in the modernization of traditional Chinese medicines (TCM), and in recent years, the fingerprinting techniques play an increasingly important role, including fingerprintings based on gas chromatography, liquid chromatography, ...

Posted in researches 5 | No Comments »

[Application of chromatography and related techniques in quality evaluation of traditional Chinese m

June 29th, 2008
[Application of chromatography and related techniques in quality evaluation of traditional Chinese medicine] Chemomics, the methodology for the study of traditional Chinese medicines (TCMs) on the substance basis, and chromatographic fingerprinting, the crucial technology of quality evaluation of TCMs, are introduced. The applications of chromatography as ...

Posted in researches 5 | No Comments »

[Prospects of the development of quality control technologies for traditional Chinese medicine]

June 29th, 2008
[Prospects of the development of quality control technologies for traditional Chinese medicine] Quality control is one of bottleneck problem limiting the applications and development of traditional Chinese medicines (TCMs). In recent years, the research on TCMs has already made a great progress. In this review, the ...

Posted in researches 5 | No Comments »

Lifestyle interventions to reduce cancer risk and improve outcomes.

June 29th, 2008
Lifestyle interventions to reduce cancer risk and improve outcomes. There are more than one half million cancer deaths in the United States each year, and one third of these deaths are attributed to suboptimal diet and physical activity practices. Maintaining a healthy weight, staying physically active ...

Posted in researches 5 | No Comments »

Update on immunizations in children and adolescents.

June 29th, 2008
Update on immunizations in children and adolescents. Over the past few years, there have been many changes to the recommendations for children and adolescents by the Advisory Committee on Immunization Practices. These include dividing the immunization schedule into two parts (i.e., ages birth to six years ...

Posted in researches 5 | No Comments »

Genetic factors in drug metabolism.

June 29th, 2008
Genetic factors in drug metabolism. Patients vary widely in their response to drugs. Having an understanding of the pharmacokinetic and pharmacodynamic properties of various medications is importantwhen assessing ethnic differences in drug response. Genetic factors can account for 20 to 95 percent of patient variability. Genetic ...

Posted in researches 5 | No Comments »

Chronic pelvic pain in women.

June 29th, 2008
Chronic pelvic pain in women. The etiology of chronic pelvic pain in women is poorly understood. Although a specific diagnosis is not found in the majority of cases, some common diagnoses include endometriosis, adhesions, irritable bowel syndrome, and interstitial cystitis. The initial history and physical examination ...

Posted in researches 5 | No Comments »

Challenging the role of calibration, validation and sensitivity analysis in relation to models of he

June 29th, 2008
Challenging the role of calibration, validation and sensitivity analysis in relation to models of health care processes. Many applications of Operational Research in the context of health care involve processes of calibration, validation and sensitivity analysis. Indeed these processes seem to have such an elevated status ...

Posted in researches 5 | No Comments »

Ultrasound waiting lists: rational queue or extended capacity?

June 29th, 2008
Ultrasound waiting lists: rational queue or extended capacity? The features and issues regarding clinical waiting lists in general and general ultrasound waiting lists in particular are reviewed, and operational aspects of providing a general ultrasound service are also discussed. A case study is presented describing a ...

Posted in researches 5 | No Comments »