Archive for May, 2008
Thursday, May 1st, 2008
Service quality for Type 2 diabetes in Australia: the patient perspective.
Aims To assess the service quality of care as perceived by people with Type 2 diabetes mellitus. Methods A cross-sectional survey using a self-administered questionnaire was carried out among members of Diabetes Australia-Queensland with Type ...
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Thursday, May 1st, 2008
An effective system of nurse-led diabetes care in rural Africa.
Aims Delivering adequate diabetes care is difficult in rural Africa because of drug and equipment shortages; as well as lack of trained medical expertise. We aimed to set up and evaluate a nurse-led protocol and education-based ...
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Thursday, May 1st, 2008
Impact of 3-year lifestyle intervention on postprandial glucose metabolism: the SLIM study.
Objective To determine the effect of a 3-year diet and exercise lifestyle intervention, based on general public health recommendations, on glucose tolerance, insulin resistance and metabolic cardiovascular risk factors in Dutch subjects with impaired ...
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Thursday, May 1st, 2008
Flexible, intensive insulin therapy and dietary freedom in adolescents and young adults with Type 1 diabetes: a prospective implementation study.
Aims To assess the outcome of a Diabetes Treatment and Teaching Programme (DTTP) on glycated haemoglobin (HbA(1c)), severe hypoglycaemia (SH) and severe ketoacidosis (SKA) in adolescents ...
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Thursday, May 1st, 2008
Insulin and glucose profiles during continuous subcutaneous insulin infusion compared with injection of a long-acting insulin in Type 2 diabetes.
Aims To compare insulin and glucose profiles during basal continuous subcutaneous infusion of a rapid-acting insulin analogue and once daily subcutaneous injection of a long-acting insulin ...
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Thursday, May 1st, 2008
Road traffic accidents and diabetes: insulin use does not determine risk.
Aims Progressive restrictions placed on insulin-treated patients with diabetes exclude them from driving group 2 and class C1 and D1 vehicles. This reflects an assumption that an increased risk of hypoglycaemia in these patients will ...
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Thursday, May 1st, 2008
Upper abdominal symptoms in patients with Type 1 diabetes: unrelated to impairment in gastric emptying caused by autonomic neuropathy.
Aims Diabetic gastroparesis is a common condition occurring in some 30-50% of patients with long-term diabetes. Some studies have found a relationship between autonomic neuropathy and diabetic ...
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Thursday, May 1st, 2008
The high prevalence of unrecognized anaemia in patients with diabetes and chronic kidney disease: a population-based study.
Background Anaemia occurs early in the course of diabetes-related chronic kidney disease (CKD). There is little evidence about the prevalence of anaemia in people with diabetes. The aim of ...
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Thursday, May 1st, 2008
Offspring birth weight, gestational age and maternal characteristics in relation to glucose status at age 53 years: evidence from a national birth cohort.
Aims We investigated pathways linking offspring birth weight to maternal diabetes risk in later life by taking into account a range of prospective ...
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Thursday, May 1st, 2008
Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli.
Escherichia coli contains a four-gene operon, pgaABCD, which encodes the proteins necessary for the synthesis of polymeric N-acetylglucosamine, or PGA. Poly-N-acetyl-glucosamine was first described in Staphylococcus aureus and Staphylococcus epidermidis and was found ...
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Thursday, May 1st, 2008
Evaluation of the diagnostic reliability of different RBC indices and formulas in the differentiation of the beta-thalassaemia minor from iron deficiency in Palestinian population.
beta-thalassaemia minor and iron deficiency are the most common causes of microcytosis and/or hypochromasia. The present study evaluates the diagnostic reliability of ...
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Thursday, May 1st, 2008
Moving EM: the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing.
In this paper, the Rapid Transfer System (RTS), an attachment to the Leica EMPACT2 high-pressure freezer, is described as a new tool for special ...
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Thursday, May 1st, 2008
Golgi apparatus studied in vitreous sections.
Cryo-electron microscopy of vitrified specimen is the method of choice to explore cellular ultrastructure at high resolution as close as possible to the native state and environment. In this study, we investigated the Golgi apparatus - the main organelle of ...
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Thursday, May 1st, 2008
Electron microscopy of the early Caenorhabditis elegans embryo.
The early Caenorhabditis elegans embryo is currently a popular model system to study centrosome assembly, kinetochore organization, spindle formation, and cellular polarization. Here, we present and review methods for routine electron microscopy and 3D analysis of the early ...
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Thursday, May 1st, 2008
Microcarriers for high-pressure freezing and cryosectioning of adherent cells.
A method is described employing microcarrier spheres of cross-linked dextran for obtaining ultra- and semithin vitreous sections from high-pressure frozen anchorage-dependent (mammalian) cells. Avoiding trypsination or scraping cells off from the culture surface, the presented approach allows ...
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Thursday, May 1st, 2008
Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography.
High-pressure freezing is the preferred method to prepare thick biological specimens for ultrastructural studies. However, the advantages obtained by this method often prove unattainable for ...
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Thursday, May 1st, 2008
Freeze-substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 degrees C.
High-pressure freezing followed by freeze substitution and plastic embedding is becoming a more widely used method for TEM sample preparation. Here, we have investigated the ...
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Thursday, May 1st, 2008
Silver enhancement of Nanogold particles during freeze substitution for electron microscopy.
Recent advances in rapid freezing and fixation by freeze substitution have allowed structural cell biologists to apply these reliable modes of sample preparation to a wide range of specimens and scientific problems. Progress in electron ...
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Thursday, May 1st, 2008
Pseudovacuoles - immobilized by high-pressure freezing - are associated with blebbing in walker carcinosarcoma cells.
By applying high pressure freezing and freeze-substitution, we observed large inclusions of homogeneous appearance in the front of locomoting Walker carcinosarcoma cells that have not been described earlier. Live cell imaging ...
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Thursday, May 1st, 2008
Evolution of residual stress and crack morphologies during 3D FIB tomographic analysis of alumina.
Three-dimensional focused ion beam (FIB) tomography is increasingly being used for 3D characterization of microstructures in the 50 nm-20 mum range. FIB tomography is a destructive, invasive process, and microstructural changes may ...
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Thursday, May 1st, 2008
Comparative study using scanning electron techniques for imaging of micro-architecture and antigen appearance.
We have used various scanning electron microscopy technologies for the analysis of structures and the appearance of antigen on eukaryotic cells. Various scanning electron microscopy including conventional scanning electron microscopy, high-resolution scanning electron ...
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Thursday, May 1st, 2008
Atomic force microscopy to characterize the molecular size of prion protein.
The conformational transition of alpha-helix-rich cellular prion protein (PrP(C)) to an isomer with high beta-sheet content is associated with transmissible spongiform encephalopathies. With the ultimate long-term goal of using imaging techniques to study PrP aggregation, ...
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Thursday, May 1st, 2008
AFM for diagnosis of nanocrystallization of steels in hardening processes.
Introduction: The aim of this study is to investigate the nanocrystallization of steels caused by the transformation from the austenitic to the martensitic phase induced by a severe plastic deformation (SPD) treatment. In this framework, we ...
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Thursday, May 1st, 2008
Correction for non-perpendicularity of beam and tilt axis in tomographic reconstructions with the IMOD package.
A correction for non-perpendicularity between the beam axis and the tilt axis in electron tomographic tilt series has been implemented in the IMOD software package and its value and limitations have ...
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Thursday, May 1st, 2008
Single wavelength micro-endoscopy in non-surgical vascular lesion diagnosis & characterization.
Malignant and dysplastic epithelial lesions have often been reported to excite vascular responses by histopathological characterization. Little is reported concerning in vivo real-time imaging of vascular patterns and flow in health or disease but the development ...
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