Archive for May, 2008

Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spiron

Thursday, May 1st, 2008

Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spironucleus vortens. ABSTRACT: BACKGROUND: Diplomonads are common free-living inhabitants of anoxic aquatic environments and are also found as intestinal commensals or parasites of a wide variety of animals. Spironucleus vortens is a putatively commensal ...

Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis.

Thursday, May 1st, 2008

Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis. ABSTRACT: BACKGROUND: The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. ...

Whole tumor antigen vaccination using dendritic cells: Comparison of RNA electroporation and pulsing

Thursday, May 1st, 2008

Whole tumor antigen vaccination using dendritic cells: Comparison of RNA electroporation and pulsing with UV-irradiated tumor cells. ABSTRACT: Because of the lack of full characterization of tumor associated antigens for solid tumors, whole antigen use is a convenient approach to tumor vaccination. Tumor RNA and apoptotic ...

Thyroglossal duct cyst carcinoma with concurrent thyroid carcinoma: a case report.

Thursday, May 1st, 2008

Thyroglossal duct cyst carcinoma with concurrent thyroid carcinoma: a case report. ABSTRACT: INTRODUCTION: Thyroglossal duct carcinoma is a very rare finding. Its presentation is similar to that of a benign cyst, which is a relatively common developmental abnormality that may manifest as a midline neck mass. ...

Validity and reliability of a home environment inventory for physical activity and media equipment.

Thursday, May 1st, 2008

Validity and reliability of a home environment inventory for physical activity and media equipment. ABSTRACT: BACKGROUND: Little is known about how the home environmental supports physical activity and screen media usage. The purpose of this study was to develop and test the reliability and validity of ...

Effectiveness of computer-tailored Smoking Cessation Advice in Primary Care (ESCAPE): a Randomised T

Thursday, May 1st, 2008

Effectiveness of computer-tailored Smoking Cessation Advice in Primary Care (ESCAPE): a Randomised Trial. ABSTRACT: BACKGROUND: Smoking remains a major public health problem; developing effective interventions to encourage more quit attempts, and to improve the success rate of self-quit attempts, is essential to reduce the numbers of ...

The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase.

Thursday, May 1st, 2008

The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase. ABSTRACT: BACKGROUND: The emergence of multi- and extensively-drug resistant Mycobacterium tuberculosis strains has created an urgent need for new agents to treat tuberculosis (TB). The enzymes of shikimate pathway are attractive targets to the development ...

Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat.

Thursday, May 1st, 2008

Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat. ABSTRACT: BACKGROUND: The Adhesion G protein-coupled receptors (GPCRs) are membrane-bound receptors with long N termini. This family has 33 members in humans. Several Adhesion GPCRs are known to have important physiological ...

Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-perform

Thursday, May 1st, 2008

Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-performance liquid chromatography. ABSTRACT: BACKGROUND: Dachengqi Tang (DT) is a common traditional Chinese medicine formula for expelling neire (\'internal heat\') in the stomach and intestines. There was no reliable analytical method available for the ...

Evaluation of biological pathways involved in chemotherapy response in breast cancer.

Thursday, May 1st, 2008

Evaluation of biological pathways involved in chemotherapy response in breast cancer. ABSTRACT: INTRODUCTION: Our goal was to examine the association between biological pathways and response to chemotherapy in estrogen receptor (ER)-positive and ER-negative breast cancers separately. METHODS: Gene set enrichment analysis including 852 predefined gene sets, ...

Polymorphism at the Apical Membrane Antigen-1 locus Reflects the World Population History of Plasmod

Thursday, May 1st, 2008

Polymorphism at the Apical Membrane Antigen-1 locus Reflects the World Population History of Plasmodium vivax. ABSTRACT: BACKGROUND: In malaria parasites (genus Plasmodium), ama-1 is a highly polymorphic locus encoding the Apical Membrane Protein-1, and there is evidence that the polymorphism at this locus is selectively maintained. ...

14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr.

Thursday, May 1st, 2008

14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr. ABSTRACT: BACKGROUND: Despite continuing advances in our understanding of AIDS pathogenesis, the mechanism of CD4+ T cell depletion in HIV-1-infected individuals remains unclear. The HIV-1 Vpr accessory protein causes cell death, likely through ...

Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infect

Thursday, May 1st, 2008

Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection. ABSTRACT: BACKGROUND: It has been well documented over past decades that interaction of pathogens with the extracellular matrix (ECM) plays a primary role in host cell attachment and invasion. Adherence to host ...

A GATA4/WT1 cooperation regulates transcription of genes required for mammalian sex determination an

Thursday, May 1st, 2008

A GATA4/WT1 cooperation regulates transcription of genes required for mammalian sex determination and differentiation. ABSTRACT: BACKGROUND: In mammals, sex determination is genetically controlled. The SRY gene, located on Y chromosome, functions as a dominant genetic switch for testis development. The SRY gene is specifically expressed in ...

Altered gene expression in the superior temporal gyrus in schizophrenia.

Thursday, May 1st, 2008

Altered gene expression in the superior temporal gyrus in schizophrenia. ABSTRACT: BACKGROUND: The superior temporal gyrus (STG), which encompasses the primary auditory cortex, is believed to be a major anatomical substrate for speech, language and communication. The STG connects to the limbic system (hippocampus and amygdala), ...

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry.

Thursday, May 1st, 2008

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry. ABSTRACT: BACKGROUND: Fitting four-parameter sigmoidal models is one of the methods established in the analysis of quantitative real-time PCR (qPCR) data. We had observed that these models are not optimal in the ...

Similarity of DMD gene deletion and duplication in the Chinese patients compared to global populatio

Thursday, May 1st, 2008

Similarity of DMD gene deletion and duplication in the Chinese patients compared to global populations. ABSTRACT: BACKGROUND: DNA deletion and duplication were determined as the major mutation underlying Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD). METHOD: Applying multiplex ligation-dependent probe amplification (MLPA), we have ...

A high sensitivity assay for the inflammatory marker C-reactive protein employing acoustic biosensin

Thursday, May 1st, 2008

A high sensitivity assay for the inflammatory marker C-reactive protein employing acoustic biosensing. ABSTRACT: C-Reactive Protein (CRP) is an acute phase reactant routinely used as a biomarker to assess either infection or inflammatory processes such as autoimmune diseases. CRP also has demonstrated utility as a predictive ...

Lack of c-kit receptor promotes mammary tumors in N-nitrosomethylurea-treated Ws/Ws rats.

Thursday, May 1st, 2008

Lack of c-kit receptor promotes mammary tumors in N-nitrosomethylurea-treated Ws/Ws rats. ABSTRACT: BACKGROUND: c-kit is expressed in various cell types during development and it has been linked to the promotion of cellular migration, proliferation and/or survival of melanoblasts, hematopoietic progenitors and primordial germ cells. Several reports ...

Distribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and wes

Thursday, May 1st, 2008

Distribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa. ABSTRACT: BACKGROUND: Knock-down resistance (kdr) to DDT and pyrethroids in the major Afrotropical vector species, Anopheles gambiae sensu stricto, is associated with two alternative point mutations at amino acid position 1014 ...

Epidemiology of foot-and-mouth disease in Landhi Dairy Colony, Pakistan, the world largest Buffalo c

Thursday, May 1st, 2008

Epidemiology of foot-and-mouth disease in Landhi Dairy Colony, Pakistan, the world largest Buffalo colony. ABSTRACT: BACKGROUND: Foot-and-mouth disease (FMD) is endemic in Pakistan and causes huge economic losses. This work focus on the Landhi Dairy Colony (LDC), located in the suburbs of Karachi. LDC is the ...

Large asymptomatic Left Atrial Myxoma with ossification: case report.

Thursday, May 1st, 2008

Large asymptomatic Left Atrial Myxoma with ossification: case report. ABSTRACT: BACKGROUND: Atrial myxomas are the most common primary cardiac tumors. They are usually small or moderate in size by the time of the diagnosis, exhibiting non specific cardiac or systemic symptoms, and are most frequently soft ...

Do quality of life, participation and environment of older adults differ according to level of activ

Thursday, May 1st, 2008

Do quality of life, participation and environment of older adults differ according to level of activity? ABSTRACT: BACKGROUND: Activity limitation is one of the most frequent geriatric clinical syndromes that have significant individual and societal impacts. People living with activity limitations might have fewer opportunities to ...

Clinical research without consent in adults in the emergency setting: a review of patient and public

Thursday, May 1st, 2008

Clinical research without consent in adults in the emergency setting: a review of patient and public views. ABSTRACT: BACKGROUND: In emergency research, obtaining informed consent can be problematic. Research to develop and improve treatments for patients admitted to hospital with life-threatening and debilitating conditions is much ...

Proteomic analysis of differential proteins in pancreatic carcinomas: effects of MBD1 knock-down by

Thursday, May 1st, 2008

Proteomic analysis of differential proteins in pancreatic carcinomas: effects of MBD1 knock-down by stable RNA interference. ABSTRACT: BACKGROUND: Methyl-CpG binding domain protein 1 (MBD1), a suppressor of gene transcription, may be involved in inactivation of tumor suppressor genes during tumorigenesis. Over-expression of MBD1 has been reported ...

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