Archive for May, 2008
Thursday, May 1st, 2008
Absence of systems consolidation of fear memories after dorsal, ventral, or complete hippocampal damage.
We examined the effects of dorsal, ventral, or complete damage to the hippocampus on long-term retention of a Pavlovian conditioned fear response to a tone and a context paired with foot shock. ...
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Thursday, May 1st, 2008
Biomolecular characterization of human glioblastoma cells in primary cultures: Differentiating and antiangiogenic effects of natural and synthetic PPARgamma agonists.
Gliomas are the most commonly diagnosed malignant brain primary tumors. Prognosis of patients with high-grade gliomas is poor and scarcely affected by radiotherapy and chemotherapy. Several studies ...
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Thursday, May 1st, 2008
Oxygen isotope analysis of carbonates in the calcite-dolomite-magnesite solid-solution by high-temperature pyrolysis: initial results.
Accurate and efficient measurement of the oxygen isotope composition of carbonates (delta(C) (18)O) based on the mass spectrometric analysis of CO(2) produced by reacting carbonate samples with H(3)PO(4) is compromised by: (1) ...
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Thursday, May 1st, 2008
Characterization of alterations of Rb2/p130 tumor suppressor in all-trans-retinoic acid resistant SK-OV3 ovarian carcinoma cells.
Rb2/p130 tumor suppressor protein regulates cell cycle progression primarily through interactions with members of the E2F family of transcription factors and repression of the transactivation of E2F target genes. In ATRA ...
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Thursday, May 1st, 2008
Is therapy with calcium and vitamin D and parathyroid autotransplantation useful in total thyroidectomy for preventing hypocalcemia?
BACKGROUND: Routine calcium and vitamin D administration and routine autotransplantation of parathyroid glands can prevent hypocalcemia after total thyroidectomy. METHODS: Routine autotransplantation of 1 or more parathyroid glands and ...
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Thursday, May 1st, 2008
Serum concentrations of laminin gamma2 fragments in patients with head and neck squamous cell carcinoma.
BACKGROUND: The laminin (LN) gamma2 chain expression has been linked to tumor invasion and prognosis. To provide a convenient clinical use, procedures that analyze LNgamma2 expression by using the serum and/or ...
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Thursday, May 1st, 2008
Myocardial function improved by electromagnetic field induction of stress protein hsp70.
Studies on myocardial function have shown that hsp70, stimulated by an increase in temperature, leads to improved survival following ischemia-reperfusion (I-R). Low frequency electromagnetic fields (EMFs) also induce the stress protein hsp70, but without elevating ...
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Thursday, May 1st, 2008
Repeatability of monolithic HPLC columns while using a flow program.
Fast HPLC methods are becoming more and more important. Using monolithic HPLC columns for fast separations, a flow program can be applied for further decrease in the total run time. An interesting issue was whether the ...
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Thursday, May 1st, 2008
Study of pepsin phosphorylation using immobilized metal affinity chromatography.
The interactions of pepsin with immobilized trivalent metal ions and the participation of the enzyme phosphate group in this process were investigated using high performance immobilized metal affinity chromatography. Two different sorbents were used: the newly prepared ...
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Thursday, May 1st, 2008
BMP signaling dynamics in embryonic orofacial tissue.
The bone morphogenetic protein (BMP) family represents a class of signaling molecules, that plays key roles in morphogenesis, cell proliferation, survival and differentiation during normal development. Members of this family are essential for the development of the mammalian orofacial ...
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Thursday, May 1st, 2008
Brachy-syndactyly caused by loss of Sfrp2 function.
Wnt signaling pathways are regulated both at the intracellular and extracellular levels. During embryogenesis, the in vivo effects of the secreted frizzled-related protein (Sfrp) family of Wnt inhibitors are poorly understood. Here, we show that inactivation of Sfrp2 results ...
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Thursday, May 1st, 2008
Two distinct amplification events of the c-myc locus in a colorectal tumour.
Southern hybridisation of genomic DNA extracted from a human primary colorectal carcinoma revealed amplification of a fragment containing the wild-type c-myc locus. Two additional rearranged DNA fragments, lying upstream of c-myc, fused to distant ...
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Thursday, May 1st, 2008
Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose.
Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. ...
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Thursday, May 1st, 2008
Investigation of interaction between human hemoglobin A(0) and platinum anticancer drugs by capillary isoelectric focusing with whole column imaging detection.
CIEF with whole column imaging detection (WCID) was used to investigate the interaction of platinum-based anticancer drugs, cis-platinum(II) diamine dichloride (cisplatin) and [SP-4-2-{1R-trans)]-(1,2-cyclohexanediamine-N,N\')[ethanedioata(2-)-O,O\']platinum (oxaliplatin), with human ...
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Thursday, May 1st, 2008
Monodisperse Gelatin Microspheres as a Drug Delivery Vehicle: Release Profile and Effect of Crosslinking Density.
Uniform gelatin microspheres (GMS) of a wet size of 100 microm in diameter were fabricated by the electric field assisted precision particle fabrication (E-PPF) method and crosslinked with different glutaraldehyde (GA) ...
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Thursday, May 1st, 2008
Structural Characterization of Mesquite (Prosopis velutina) Gum and its Fractions.
Structural and physicochemical characteristics of mesquite gum (from Prosopis velutina) were investigated using FT-IR spectroscopic, mass spectrometric and chromatographic methods. Four fractions (F-I, F-IIa, F-IIb and F-III) were isolated by hydrophobic interaction chromatography. The samples were ...
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Thursday, May 1st, 2008
Macrocyclic polyamine-modified poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith for capillary electrochromatography.
1,4,10,13,16-Pentaazatricycloheneicosane-9,17-dione (macrocyclic polyamine)-modified polymer-based monolithic column for CEC was prepared by ring opening reaction of epoxide groups from poly(glycidyl methacrylate-co-ethylene dimethacrylate) (GMA-co-EDMA) monolith with macrocyclic polyamine. Conditions such as reaction time and concentration of macrocyclic polyamine for ...
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Thursday, May 1st, 2008
Fabrication and integration of planar electrodes for contactless conductivity detection on polyester-toner electrophoresis microchips.
In this report, we describe the microfabrication and integration of planar electrodes for contactless conductivity detection on polyester-toner (PT) electrophoresis microchips using toner masks. Planar electrodes were fabricated by three simple steps: ...
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Thursday, May 1st, 2008
A novel strategy for avian species identification by cytochrome b gene.
We report a DNA-based test that can be applied to any avian species so that the amplicon can be used in species identification. The need for the test arose from the requirement to enforce the ...
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Thursday, May 1st, 2008
Comprehensive proteome analysis of mouse liver by ampholyte-free liquid-phase isoelectric focusing.
In this study, ampholyte-free liquid-phase IEF (LIEF) was combined with narrow pH range 2-DE and SDS-PAGE RP-HPLC for comprehensive analysis of mouse liver proteome. Because LIEF prefractionation was able to reduce the complexity of the ...
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Thursday, May 1st, 2008
Microchip analysis of plant glucosinolates.
We describe a new and selective analytical method for the separation and quantitation of plant glucosinolates. The new method, which utilizes microchip CE (mu-CE) with fluorescence detection, circumvents the multistep procedures characteristic of conventional methods. Glucosinolates form charge transfer complexes with ...
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Thursday, May 1st, 2008
Novel approaches to predict the retention of histidine-containing peptides in immobilized metal-affinity chromatography.
The new method lazy learning method-local lazy regression (LLR) was first used to model the quantitative structure-retention relationship (QSRR) for predicting and explaining the retention behaviors of peptides in the nickel column in ...
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Thursday, May 1st, 2008
The up-regulation of 14-3-3 proteins in Smad4 deficient epidermis and hair follicles at catagen.
Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows ...
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Thursday, May 1st, 2008
Identification of peptidases in Nicotiana tabacum leaf intercellular fluid.
Peptidases in the extracellular space might affect the integrity of recombinant proteins expressed in, and secreted from, plant cells. To identify extracellular peptidases, we recovered the leaf intercellular fluid from Nicotiana tabacum plants by an infiltration-centrifugation method. ...
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Thursday, May 1st, 2008
Self-Assembly of Single-Stranded RNA on Carbon Nanotube: Polyadenylic Acid to Form a Duplex Structure.
All messenger-RNA (mRNA) molecules in eukaryotic cells have a polyadenylic acid [poly(rA)] tail at the 3\'-end and human poly(rA) polymerase (PAP) has been considered as a tumor-specific target. A ligand that is ...
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