Archive for the ‘researches 3’ Category
Sunday, March 16th, 2008
[Initial functional analysis of the promoter region and coding region of Pib gene in transgenic rice.]
The promoter region and intact coding region of Pib gene (9.9 kb) was inserted into the downstream of CaMV 35S promoter in a binary vector pPZP2Ha3(+), resulting a plasmid pNAR701. ...
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Sunday, March 16th, 2008
[Cloning and sequence analysis of TSEG-1, a novel gene specifically expressed in mouse testis.]
The expressed sequence tags (ESTs) of normal mouse testis were obtained from online EST database ZooDDD. Their highly homologous EST sequences were found through the dbEST database to construct contigs, and spliced ...
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Sunday, March 16th, 2008
[Analysis of genetic structure of mirror carp population and correlation of microsatellite markers and economic traits.]
The genetic structure of one breeding population of mirror carp(Cyprinus carpio L.) was analysed using thirty-five polymorphic microsatellite markers. The effective number of alleles (Ae), observed heterozygosity (Ho), expected heterozygosity ...
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Sunday, March 16th, 2008
[Cloning of gene fragment of estrogen receptor-beta and its expression in mouse embryo.]
In order to study the expression and regulation effects of estrogen receptor-beta (ERbeta) in the development of mouse embryo, the primer of ERbeta was designed, the ERbeta fragment was first obtained by RT-PCR ...
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Sunday, March 16th, 2008
[Generation of bone morphogenetic protein 4 conditional RNA inter-ference mice.]
To explore bone morphogenetic protein 4 (BMP4) function in the developing bone, a BMP4 conditional RNA interference (CRNAi) vector was constructed based on the pBSK/U6 vector with a LoxPneo cassette. The transgene fragment targeting bmp4 was ...
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Sunday, March 16th, 2008
[Association of polymorphisms of DGAT2 gene with growth traits in Jiaxian red cattle.]
The polymorphisms of DGAT2 gene in 92 Jiaxian red cattle were analyzed using PCR-RFLP method. Results showed that the PCR products digested with TaqI demonstrated polymorphisms, and showed two kinds of genotype: AA ...
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Sunday, March 16th, 2008
[Expression profiles of the extracellular matrix-associated genes during rat liver regeneration.]
It has been well known that the extracellular matrix (ECM) plays an important role in cell polarization, cell adhesion, cell proliferation, morphogenesis and differentiation. For the sake of the further in-depth investigation of the changes ...
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Sunday, March 16th, 2008
[African origin of Chinese domestic donkeys.]
Analysis of the 367 mtDNA D-loop sequences (of which 241 sequences were collected from literature) of 399 bp in 13 Chinese domestic donkey breeds revealed 96 different haplotypes with 57 polymorphic sites. The haplotype diversity and the nucleotide diversity were ...
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Sunday, March 16th, 2008
[Distribution of HIV/AIDS associated CCR2-64allele of 14 Chinese ethnic populations in South China.]
The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR2-64 alleles in 14 Chinese populations in South China. The blood samples include 791 unrelated healthy individuals. Whole ...
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