Archive for the ‘researches 3’ Category
Sunday, March 16th, 2008
Analysis of nucleotide binding to p97 reveals the properties of a tandem AAA hexameric ATPase.
p97, an essential chaperone in endoplasmic reticulum associated degradation and organelle biogenesis, contains two AAA domains (D1 and D2) and assembles as a stable hexamer. We present a quantitative analysis of ...
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Sunday, March 16th, 2008
Structural and thermodynamic analyses of solute-binding protein from bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I.
Recently, a gene cluster involving a phosphorylase specific for lacto-N-biose I (LNB; Galbeta1-3GlcNAc) and galacto-N-biose (GNB; Galbeta1-3GalNAc) has been found in Bifidobacterium longum. We showed that the solute-binding ...
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Sunday, March 16th, 2008
Notch1 upregulation and signaling involved in mycobacterium bovis BCG induced SOCS3 expression in macrophages.
Suppressor of cytokine signaling (SOCS) 3 is a critical negative regulator of cytokine signaling and is induced by Mycobacterium bovis Bacille Calmette-Guérin (M. bovis BCG) in mouse macrophages. However, little is known ...
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Sunday, March 16th, 2008
Manganese binding to the prion protein.
There is considerable evidence that the prion protein binds copper. However, there have also been suggestions that PrP binds manganese. We used isothermal titration calorimetry to identify the manganese binding sites in wild-type mouse PrP. The protein showed two manganese ...
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Sunday, March 16th, 2008
Role of cadherin-mediated cell-cell adhesion in pancreatic exocrine-to-endocrine transdifferentiation.
Although pancreatic exocrine acinar cells have the potential to transdifferentiate into pancreatic endocrine cells, the mechanisms are poorly understood. Here we report that intracellular signaling pathways, including those involving MAPK and PI3-kinase, are activated by enzymatic dissociation ...
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Sunday, March 16th, 2008
Synergistic collagenase expression and cartilage collagenolysis are phosphatidylinositol 3\'-kinase/AKT signalling-dependent.
The phosphatidylinositol 3\'-kinase (PI3K) signalling pathway has emerged as a major regulator of cellular functions and has been implicated in several pathologies involving remodelling of extracellular matrix (ECM). The end stage of inflammatory joint diseases is ...
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Sunday, March 16th, 2008
Biosynthetic exchange of bromide for chloride and strontium for calcium in the Photosystem II oxygen-evolving enzyme.
The active site for water oxidation in Photo-system II goes through five sequential oxida-tion states (S0 to S4) before O2 is evolved. It consists of a Mn4Ca-cluster close to a ...
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Sunday, March 16th, 2008
Tumor suppressor cylindromatosis (CYLD) acts as a negative regulator for Streptococcus pneumoniae-induced NFAT signaling.
Gram-positive bacterium Streptococcus pneumoniae (S. pneumoniae) is an important human pathogen that colonizes the upper respiratory tract and also the major cause of morbidity and mortality worldwide. S. pneumoniae causes invasive diseases ...
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Sunday, March 16th, 2008
Calcium ions are involved in the unusual red-shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purple sulfur bacterium thermochromatium tepidum.
Thermophilic purple sulfur bacterium, Thermochromatium tepidum, can grow at temperatures up to 58 degrees C and exhibits an unusual Qy absorption ...
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Sunday, March 16th, 2008
Protein kinase D-dependent phosphorylation and nuclear export of histone deacetylase 5.
Vascular endothelial growth factor (VEGF) is essential for normal and pathological angiogenesis. However, the signaling pathways linked to gene regulation in VEGF-induced angiogenesis are not fully understood. Here we demonstrate a critical role of protein ...
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Sunday, March 16th, 2008
Subunit alpha facilitates aqueous access to a membrane-embedded region of subunit c in Escherichia coli F1Fo ATP synthase.
Rotary catalysis in F1Fo ATP synthase is powered by proton translocation through the membrane embedded Fo sector. Proton binding and release occurs in the middle of the membrane ...
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Sunday, March 16th, 2008
Functional characterization of murine interferon regulatory factor (IRF-5) and its role in the innate antiviral response.
While the role of human IRF5 in antiviral and inflammatory responses in vitro has been well characterized, much remains to be elucidated about murine IRF-5. Murine IRF-5, unlike the heavily ...
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Sunday, March 16th, 2008
Annexin A2 interactions with Rab14 in alveolar type II cells.
Annexin A2, a calcium-dependent phospholipid-binding protein, is abundantly expressed in alveolar type II cells where it plays a role in lung surfactant secretion. Nevertheless, little is known about the details of its cellular pathways. To identify ...
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Sunday, March 16th, 2008
Design of an active ultra-stable single-chain insulin analog. Synthesis, structure, and therapeutic implications.
Single-chain insulin analogs (SCIs) provide insight into the interrelation of hormone structure, function, and dynamics. Although compatible with wild-type structure, short connecting segments (< 3 residues) prevent induced fit on receptor binding and ...
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Sunday, March 16th, 2008
Identification of novel isoforms of mouse L-selectin with different carboxy-terminal tails.
The leukocyte adhesion molecule L-selectin mediates the recruitment of lymphocytes to secondary lymphoid organs and is involved in the accumulation of neutrophils at sites of inflammation. In this study, we report the identification of novel ...
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Sunday, March 16th, 2008
Regulation of the Drosophila HIF-{alpha} protein Sima by CRM1-dependent nuclear export.
HIF-alpha proteins are regulated by oxygen levels through several different mechanisms that include protein stability, transcriptional co-activator recruitment and subcellular localization. It was previously reported that these transcription factors are mainly nuclear in hypoxia and ...
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Sunday, March 16th, 2008
Signaling through ShcA is required for TGF-{beta} and Neu/ErbB-2 induced breast cancer cell motility and invasion.
Cooperation between the Neu/ErbB-2 and TGF-beta signaling pathways enhances the invasive and metastatic capabilities of breast cancer cells; however, the underlying mechanisms mediating this synergy have yet to be fully ...
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Sunday, March 16th, 2008
Redundant roles of Tead1 and Tead2 in notochord development and the regulation of cell proliferation and survival.
Four members of the TEAD/TEF family of transcription factors are expressed widely in mouse embryos and adult tissues. Although in vitro studies have suggested various roles for TEAD proteins, ...
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Sunday, March 16th, 2008
PTEN Nuclear Localization Is Regulated by Oxidative Stress and Mediates p53-Dependent Tumor Suppression.
The tumor suppressor gene PTEN (phosphatase and tensin homologue deleted on chromosome 10) is frequently mutated or deleted in various human cancers. PTEN localizes predominantly to the cytoplasm and functions as a lipid ...
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Sunday, March 16th, 2008
A conserved phenylalanine of motif IV in superfamily 2 helicases is required for cooperative, ATP-dependent, binding of RNA substrates in DEAD-box proteins.
We have identified a highly conserved phenylalanine in motif IV of the DEAD-box helicases that is important for their enzymatic activities. In vivo analyses ...
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Sunday, March 16th, 2008
Eukaryotic wobble uridine modifications promote a functionally redundant decoding system.
The translational decoding properties of tRNAs are modulated by naturally occurring modifications of their nucleosides. Uridines located at the wobble position (nucleoside 34) in eukaryotic cytoplasmic tRNAs often harbor a 5-methoxycarbonylmethyl (mcm(5)) or a 5-carbamoylmethyl (ncm(5)) ...
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Sunday, March 16th, 2008
TGF{alpha}-Stimulated Secretion of HSP90{alpha}: Using LRP-1/CD91 Receptor To Promote Human Skin Cell Migration Against TGF{beta}-Rich Environment In Wound Healing.
Jump-starting and subsequently maintaining epidermal and dermal cell migration are essential for skin wound healing. These events are often disrupted in non-healing wounds, causing patient morbidity and ...
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Sunday, March 16th, 2008
Different Mechanisms for Pseudouridine Formation in Yeast 5S and 5.8S Ribosomal RNAs.
Selection of sites for pseudouridylation in eukaryotic cytoplasmic ribosomal RNA (rRNA) occurs by base-pairing with specific guide sequences within the RNA components of box H/ACA small nucleolar ribonucleoproteins (snoRNPs). 44 of the 46 pseudouridines ...
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Sunday, March 16th, 2008
Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits.
BUD23 was identified from a bioinformatics analysis of yeast genes involved in ribosome biogenesis. Deletion of BUD23 leads to severely impaired growth, reduced levels of the small (40S) ribosomal subunit ...
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Sunday, March 16th, 2008
Abnormal sympatho-adrenal development and systemic hypotension in PHD3-/- mice.
Cell culture studies have implicated the oxygen-sensitive HIF prolyl hydroxylase PHD3 in the regulation of neuronal apoptosis. To better understand this function in vivo, we have created PHD3-/- mice and analysed the neuronal phenotype. Reduced apoptosis in ...
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