Archive for the ‘researches 3’ Category
Saturday, March 29th, 2008
Coming soon to your clinic: high-quality ART.
The concept of \'patient-friendly\' medically assisted reproduction includes a robust set of clinical practice principles, to improve the quality of subfertility care. This concept is an important move away from the sole focus on effectiveness and high pregnancy rates ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Structural properties of AMP-activated protein kinase. Dimerization, molecular shape, and changes upon ligand binding.
Heterotrimeric AMP-activated protein kinase (AMPK) is crucial for cellular energy homeostasis of eukaryotic cells and organisms. Recently, we developed a bacterial co-expression strategy for functionally intact full-length mammalian AMPK with a His-tag. ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Association of protein phosphatase 1gamma 1 with spinophilin suppresses phosphatase activity in a Parkinson\'s disease model.
Sustained nigrostriatal dopamine depletion increases the serine/threonine phosphorylation of multiple striatal proteins that play a role in corticostriatal synaptic plasticity, including Thr(286) phosphorylation of CaMKIIalpha. Mechanisms underlying these changes are ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Cul2 is required for the activity of hypoxia-inducible factor and vasculogenesis.
CULLIN 2 (CUL2) is a component of the ElonginB/C-CUL2-RBX-1-Von Hippel-Lindau (VHL) tumor suppressor complex that ubiquitinates and degrades hypoxia inducible factor a (HIFa). HIFa is a transcription factor that mediates the expression of hypoxia-sensitive genes, ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Regulation of Proline-Rich Akt Substrate of 40 kDa function by mammalian target of rapamycin complex 1 -mediated phosphorylation.
The rapamycin-sensitive mammalian target of rapamycin (mTOR) complex 1 (mTORC1) contains mTOR, raptor, mLST8 and PRAS40 (Proline-Rich Akt Substrate of 40 kDa). PRAS40 functions as a negative regulator ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Reaction mechanism and structural model of ADP -forming acetyl-coa synthetase from the hyperthermophilic archaeon pyrococcus furiosus: Evidence for a second active site histidine residue.
In archaea, acetate formation and ATP synthesis from acetyl-CoA is catalysed by an unusual ADP-forming acetyl-CoA synthetase (ACD) catalyzing the formation of ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Molecular machinery of mitochondrial fusion and fission.
Mitochondria generate energy by oxidative phosphorylation, play a crucial role in iron-sulphur cluster assembly, and participate in intermediary metabolism, calcium signalling and apoptosis. They are bounded by a double membrane and contain about 800 (yeast) to 1500 (human) different ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Identification of extracellular-regulated kinase 1/2 and p38MAPK as regulators of human sperm motility and acrosome reaction and as predictors of poor spermatozoa quality.
Mature spermatozoa acquire progressive motility only after ejaculation. Their journey in the female reproductive tract includes also suppression of progressive motility, reactivation, capacitation ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Loss of prostaglandin F2alpha, but not thromboxane, responsiveness in pregnant human myometrium during labour.
Prostaglandins (PG) E2, PGF2alpha and thromboxane (TX) mediate uterine contractility by targeting prostonoid EP, FP and TP receptors respectively. The aim of this study was to elucidate the function of these receptors ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
The augmenting effect on insulin secretion by oral versus intravenous glucose is exaggerated by high-fat diet in mice.
To study whether the incretin effect is involved in adaptively increased insulin secretion in insulin resistance, glucose was infused at a variable rate to match glucose levels after ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Involvement of bone morphogenetic protein-4 in GH regulation by octreotide and bromocriptine in rat pituitary GH3 cells.
Here we investigated roles of the pituitary bone morphogenetic protein (BMP) system in modulating GH production regulated by a somatostatin analog, octreotide (OCT) and a dopamine agonist, bromocriptine (BRC) ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Lacking thyroid hormone receptor beta gene does not influence alterations in peripheral thyroid hormone metabolism during acute illness.
The downregulation of liver deiodinase type 1 (D1) is supposed to be one of the mechanisms behind the decrease in serum tri-iodothyronine (T3) observed during non-thyroidal illness (NTI). ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
P70 S6 kinase negatively regulates fibroblast growth factor 2-stimulated interleukin-6 synthesis in osteoblasts: function at a point downstream from protein kinase C.
We have previously reported that protein kinase C negatively regulates basic fibroblast growth factor (FGF-2)-stimulated synthesis of interleukin-6 (IL-6), a potent bone resorptive agent, ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Interaction between pro-inflammatory and anti-inflammatory cytokines in insulin-producing cells.
Pro-inflammatory cytokines cause beta-cell dysfunction and death. The aim of this study was to investigate the interactions between different pro- and anti-inflammatory cytokines and their effects on apoptotic beta-cell death pathways. Insulin-producing RINm5F cells were exposed to ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Protein kinase C alpha modulates liver X receptor alpha transactivation.
Liver X receptor alpha (LXRalpha), an oxysterol-activated nuclear hormone receptor, regulates the expression of genes involved in lipid and cholesterol homeostasis and inflammation. We show here that transactivation by LXRalpha in monkey kidney COS-1 (Cos-1) cells ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Comparison of expressed human and mouse sodium/iodide symporters reveals differences in transport properties and subcellular localization.
The active transport of iodide from the bloodstream into thyroid follicular cells is mediated by the Na+/I- symporter (NIS). We studied mouse NIS (mNIS) and found that it catalyzes iodide ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Adipose tissue loss in adjuvant arthritis is associated with a decrease in lipogenesis, but not with an increase in lipolysis.
Adjuvant-induced arthritis is a model of rheumatoid arthritis that induces cachexia. In other cachectic situations, there is an increase in lipolysis resulting in a loss of ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Angiotensin II activates myostatin expression in cultured rat neonatal cardiomyocytes via p38 MAP kinase and myocyte enhance factor 2 pathway.
Angiotensin II (AngII) plays a critical role in cardiac remodeling and promotes cardiac myocyte hypertrophy. Myostatin, a negative regulator of muscle growth, is increased in hypertrophied ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Early blood pressure-independent cardiac changes in diabetic rats.
Cardiac remodeling is a key event in both diabetic and hypertensive heart diseases. In the present study, we investigated early myocardial changes in an animal model, the male Sabra rat model (SBH/y) of salt-induced hypertension-rendered diabetic with streptozotocin. ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Comparative expression analysis of the renin-angiotensin system components between white and brown perivascular adipose tissue.
Recent studies have demonstrated that the rat adipose tissue expresses some of the components necessary for the production of angiotensin II (Ang II) and the receptors mediating its actions. The aim ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Tri-iodothyronine treatment differently affects liver metabolic response and oxidative stress in sedentary and trained rats.
We investigated whether swim training modifies the effects of tri-iodothyronine (T3) treatment on the metabolic response and oxidative damage of rat liver. Respiratory capacities, oxidative damage, levels of antioxidants, and susceptibility ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
FSH-induced phosphoprotein phosphatase 2A-mediated deactivation of particulate phosphodiesterase-4 activities is abolished after alteration in proteoglycan synthesis in immature rat Sertoli cells.
Cessation of rat testicular Sertoli cells proliferation around days 15-20 post partum is associated in vitro with the highest rise in rolipram-sensitive cAMP-catabolizing phosphodiesterase-4 activities ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Pathways involved in testicular germ cell apoptosis in immature rats after FSH suppression.
FSH is a key regulator of testis function, required for the establishment of full complements of Sertoli and germ cells during postnatal testis development and for the maintenance of spermatogenesis in the adult. ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles.
Androgens stimulate many hair follicles to alter hair colour and size via the hair growth cycle; in androgenetic alopecia tiny, pale hairs gradually replace large, pigmented ones. Since stem cell factor (SCF) is important in ...
Posted in researches 3 | No Comments »
Saturday, March 29th, 2008
Changes in angiotensin II type 1 receptor signalling pathways evoked by a monoclonal antibody raised to the N-terminus.
The extracellular N-terminus of G-protein-coupled receptors may be involved in signalling events. We examined this in the angiotensin II type 1 receptor (AT1-R) using monoclonal antibody 6313/G2, raised ...
Posted in researches 3 | No Comments »