Archive for the ‘for377cat’ Category
Friday, June 27th, 2008
Enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody as a new tool to detect Sudan dyes and Para red.
To set up an immunoassay-based method to detect Sudan dyes and Para red, we generated a monoclonal antibody (Mab) using a specially designed carboxyl derivative of ...
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Friday, June 27th, 2008
Bioseparation of recombinant cellulose-binding module-proteins by affinity adsorption on an ultra-high-capacity cellulosic adsorbent.
Low-cost protein purification methods are in high demand for mass production of low-selling price enzymes that play an important role in the upcoming bioeconomy. A simple protein purification method was developed based on ...
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Friday, June 27th, 2008
Carrier-mediated liquid phase microextraction coupled with high performance liquid chromatography for determination of illicit drugs in human urine.
A new method was developed for the analysis of illicit drugs in human urine by coupling carrier-mediated liquid phase microextraction (LPME) to high performance liquid chromatography (HPLC). By ...
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Friday, June 27th, 2008
Ionic liquid-based miniature electrochemical sensors for the voltammetric determination of catecholamines.
Screen-printed electrodes modified with carbon paste that consisted of graphite powder dispersed in ionic liquids (IL) were used for the electrochemical determination of dopamine, adrenaline and dobutamine in aqueous solutions by means of cyclic voltammetry. ...
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Friday, June 27th, 2008
Dual fluorescence/contactless conductivity detection for microfluidic chip.
A new dual detection system for microchip is reported. Both fluorescence detector (FD) and contactless conductivity detector (CCD) were combined together and integrated on a microfluidic chip. They shared a common detection position and responded simultaneously. A blue light-emitting ...
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Friday, June 27th, 2008
Target transform fitting in a voltammetric study of metal complexation.
In this work target transform fitting (TTF) is proposed as a hard-model based data analysis method to analyze differential pulse polarograms recorded in a successive metal complexation system. In such cases, equations for both complexation equilibria ...
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Friday, June 27th, 2008
Multivariate characterisation and quantitative structure-property relationship modelling of nitroaromatic compounds.
A multivariate model to characterise nitroaromatics and related compounds based on molecular descriptors was calculated. Descriptors were collected from literature and through empirical, semi-empirical and density functional theory-based calculations. Principal components were used to describe the ...
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Friday, June 27th, 2008
Mercury speciation in environmental solid samples using thermal release technique with atomic absorption detection.
A sensitive and very simple method for determination of mercury species in solid samples has been developed involving thermal release analysis in combination with atomic absorption (AAS) detection. The method allows determination ...
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Friday, June 27th, 2008
Rare earth elements determined in Antarctic ice by inductively coupled plasma--time of flight, quadrupole and sector field-mass spectrometry: An inter-comparison study.
Inductively coupled plasma mass spectrometry (ICP-MS) is a suitable tool for multi-element analysis at low concentration levels. Rare earth element (REE) determinations in standard reference ...
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Friday, June 27th, 2008
Novel analytical methods for characterising binding media and protective coatings in artworks.
Since the first reported analytical studies and technical examinations of art and archaeological objects conducted in the late 18th century, analytical techniques and methods applied to the study of artworks have constantly grown. Among ...
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Friday, June 27th, 2008
Applications of aptamers in cancer cell biology.
Identifying cells associated with specific disease states is critically important for the early detection and diagnosis of cancer. To facilitate this task, molecular probes, which bind biomarkers that are either specifically or differentially expressed in diseased cells relative to ...
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Friday, June 27th, 2008
Aging markers from bottled red wine aged with chips, staves and barrels.
This paper shows the results of the experiments carried on Spanish red wines aged through alternative as well as traditional oak barrel systems, i.e., chips stainless steel tanks and staves stainless steel tanks, at ...
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Friday, June 27th, 2008
Free and hydrolytically released volatile compounds of Vitis vinifera L. cv. Fiano grapes as odour-active constituents of Fiano wine.
Grape-derived volatile compounds, including those released from odourless glycosidic precursor present in the grape, are strongly associated with the varietal aroma characteristic of non-aromatic wines. In this ...
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Friday, June 27th, 2008
Measurement of dissolved oxygen during red wines tank aging with chips and micro-oxygenation.
Nowadays, micro-oxygenation is a very important technique used in aging wines in order to improve their characteristics. The techniques of wine tank aging imply the use of small doses of oxygen and the ...
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Friday, June 27th, 2008
Assessing the impact of temperature on grape phenolic metabolism.
This study assessed the impact of fruit temperature on the phenolic metabolism of grape berries (Vitis vinifera L. cv. Merlot) grown under field conditions with controlled exposure to sunlight. Individual cluster temperatures were manipulated in situ. Diurnal ...
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Friday, June 27th, 2008
Relevance of chromatographic efficiency in varietal authenticity verification of red wines based on their anthocyanin profiles: Interference of pyranoanthocyanins formed during wine ageing.
The determination of the grape variety of aged red wines based on the ratio of acetylated and coumaroylated anthocyanins (R(ac/coum)) can be influenced ...
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Friday, June 27th, 2008
Understanding the contribution of ascorbic acid to the pigment development in model white wine systems using liquid chromatography with diode array and mass spectrometry detection techniques.
The present study investigated the contribution of ascorbic acid to the formation of coloured species in model white wine systems ...
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Friday, June 27th, 2008
Hyphenated gas chromatography-mass spectrometry analysis of 3-mercaptohexan-1-ol and 3-mercaptohexyl acetate in wine: Comparison with results of other sampling procedures via a robust regression.
This work describes a new purge and trap gas chromatography electron impact mass spectrometry (PT-GC-EIMS) method for quantifying 3-mercaptohexan-1-ol (3-MH) and 3-mercaptohexyl acetate ...
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Friday, June 27th, 2008
Kinetics and stability of the mixing flow patterns found in champagne glasses as determined by laser tomography techniques: likely impact on champagne tasting.
Laser tomography techniques were used in order to make visible the flow patterns induced by ascending bubbles in flutes poured with champagne. The ...
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Friday, June 27th, 2008
Identification of S-methylmethionine in Petit Manseng grapes as dimethyl sulphide precursor in wine.
A procedure for the extraction of free amino acids was applied to isolate S-methylmethionine (SMM) from late harvest Petit Manseng grapes. Grapes were destemmed and crushed, and the obtained clarified must was percolated ...
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Friday, June 27th, 2008
Varietal discrimination of Australian wines by means of mid-infrared spectroscopy and multivariate analysis.
This study outlines the use of mid-infrared (MIR) spectroscopy combined with principal component analysis (PCA) and linear discriminant analysis (LDA) for the varietal classification of commercial red and white table wines. Three red ...
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Friday, June 27th, 2008
Front face fluorescence spectroscopy and visible spectroscopy coupled with chemometrics have the potential to characterise ripening of Cabernet Franc grapes.
The potential of front-face spectroscopy for grape ripening dates discrimination was investigated on Cabernet Franc grapes from three parcels located on the Loire Valley and for ...
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Friday, June 27th, 2008
Use of direct headspace-mass spectrometry coupled with chemometrics to predict aroma properties in Australian Riesling wine.
The aim of this study was to investigate the potential use of a direct headspace-mass spectrometry electronic nose instrument (MS e_nose) combined with chemometrics as rapid, objective and low cost ...
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Friday, June 27th, 2008
NMDA enhances stretching-induced differentiation of osteobalsts through the ERK1/2 signaling pathway.
The neurotransmitter N-methyl-d-aspartate (NMDA) and stretching both increase Ca(2+) influx in osteoblastic cells. We postulated that NMDA would enhance the osteoblastic cell\'s response to stretching. The goal of this study was to investigate, in the ...
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Friday, June 27th, 2008
Solubilization of membrane protein complexes for blue native PAGE.
Blue native PAGE is an electrophoretic technique for high-resolution separation of membrane proteins. The method has been proven especially useful for investigation of native protein complexes enabling a characterization of potential protein-protein interactions in the context of ...
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