Archive for the ‘for26cat’ Category
Saturday, June 28th, 2008
Roles of embryonic and adult lymphoid tissue inducer cells in primary and secondary lymphoid tissues.
The nomenclature \"embryonic lymphoid tissue inducer (LTi) cell\" reflects the fundamental role of the cell in secondary lymphoid tissue organization. In addition, it is equally important in primary lymphoid tissue development ...
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Saturday, June 28th, 2008
Hormone-dependent aging problems in women.
One of the major social issues nowadays is the aging society. Korea is already an aging society, and 63 cities and districts are ultra-aged societies where the rate of people older than 65 yr exceeds 20%. Among them, more than 67% ...
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Saturday, June 28th, 2008
High-Density culture of mouse-human hybridoma in serum-free defined medium.
Mouse-human hybridoma 4H11 cells producing anti-Pseudomonas sp. monoclonal antibody (IgA) grew in a serum-free medium supplemented with insulin, transferrin, ethanolamine, and selenite (ITES). The hybridoma could be applied to high-density culture in a serum-free medium supplemented with ...
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Saturday, June 28th, 2008
Specific and nonspecific glucanases from Trichoderma viride.
Six endoglucanases (Endo I, II, III, IV, V, and VI), three exoglucanases (Exo I, II, and III), and a beta-glucosidase (beta-gluc I) isolated from a commercial cellulase preparation of Trichoderma viride origin were examined as to their activities on ...
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Saturday, June 28th, 2008
Mathematical modeling for mixed culture growth of two bacterial populations with opposite substrate preferences.
An unstructured mathematical model is proposed for mixed culture growth of two different bacterial species that exhibit \"opposite\" substrate preferences in response to the \"same\" environmental conditions. The model incorporates enzymatic control ...
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Saturday, June 28th, 2008
In situ extractive fermentation of acetone and butanol.
The productivity of the acetone-butanol fermentation was increased by continuously removing acetone and butanol from the fermentation broth during fed-batch culture. Whole broth containing viable cells of Clostridium acetobutylicum was cycled to a Karr reciprocating plate extraction column ...
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Saturday, June 28th, 2008
Effect of pH on growth of mixed cultures in batch reactor.
This work has studied the effect of pH on specific growth rate mu, yield factor Y, and specific substrate consumption rate U for two mixed microbial populations (sludge A and B) in a batch reactor ...
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Saturday, June 28th, 2008
Kinetics and regulation of beta-lactamase formation in methylotrophic bacterium.
The regulation and kinetics of formation of an inducible, cell-bound oxacillin-hydrolyzing beta-lactamase (M-OXA) by a methanol-grown Pseudomonas strain were investigated in batch, chemostat, and two-stage continuous cultures. The extent to which enzyme production occurs declines at increased ...
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Saturday, June 28th, 2008
Theoretical study of tryptophan operon: Application in microbial technology.
A model for the tryptophan operon is formulated based on the genetic and biophysical data available on the structure of the operon and the nature of interactions between the represser and its ligands. Studies have been done, ...
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Saturday, June 28th, 2008
Escherichia coil growth dynamics: A three-pool biochemically based description.
A three-pool growth model of an individual Escherichia coli cell is described herein. The model is based on a previously developed chemically structured complex single cell growth model. The reduction in model complexity and the identification of ...
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Saturday, June 28th, 2008
Bioflocculation as a microbial response to substrate limitations.
Previous theories of nutrient supply to microbial floes assumed that transport within the flocs was by molecular diffusion, and they predict that overall nutrient uptake is reduced in floes compared to dispersed cells. Calculations, supported by recent advances ...
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Saturday, June 28th, 2008
Limitation of growth rate by two complementary nutrients: Some elementary but neglected considerations.
Experimental data in the literature show that the yield of biomass from a particular nutrient when that nutrient limits growth rate is often significantly different than the yield from the nutrient when some ...
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Saturday, June 28th, 2008
Alum flocculation and bioflocculation of activated sludge for vacuum filtration.
This study investigated the relationship between sludge loading rate, COD-to-nitrogen ratio of influent waste, and maximum difference in specific resistance as a result of chemical conditioning (DeltaZ). It also related DeltaZ to sludge carbohydrate content, protein ...
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Saturday, June 28th, 2008
Thermochemical pretreatment of lignocellulose to enhance methane fermentation: II. Evaluation and application of pretreatment model.
A model was developed and evaluated as a tool for predicting the formation of soluble products from staged thermochemical treatment of lignocellulosic materials under acidic conditions typical of autohydrolysis. The model ...
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Saturday, June 28th, 2008
Thermochemical pretreatment of lignocellulose to enhance methane fermentation: I. Monosaccharide and furfurals hydrothermal decomposition and product formation rates.
Over a pH range 1-4 and temperatures from 170 to 230 degrees C, the decomposition rates of xylose, gaiactose, mannose, glucose, 2-furfural, and 5-hydroxymethyl-2-furfural (5-HMF) were pseudo first ...
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Saturday, June 28th, 2008
Production and application of methylotrophic yeast Pichia pastoris.
Pichia pastoris is a methylotrophic yeast that makes use bf the enzyme alcohol oxidase to catalyze the first step of the dissimilatory pathway that enables it to grow on methanol. Because of its stability and low substrate specificity, ...
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Saturday, June 28th, 2008
Revised enzyme synthesis rate expression in cybernetic models of bacterial growth.
A revised enzyme synthesis rate expression for cybernetic models of bacterial growth is presented. The rate expression, which is comprised of inducible and constitutive contributions, provides for a basal enzyme level that is necessary to ...
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Saturday, June 28th, 2008
Kinetic analysis of bioconversion of cellulose in attrition bioreactor.
The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deacti-vation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of ...
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Saturday, June 28th, 2008
Concentration multiplicity in a draft tube fluidized-bed bioreactor involving two limiting substrates.
Concentration multiplicity in a two-phase or three-phase draft tube fluidized-bed bioreactor containing biofloc particles is studied. The kinetics of biological reactions considered involve two limiting substrates. The necessary and sufficient conditions for concentration multiplicity ...
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Saturday, June 28th, 2008
New instrument for on-line viscosity measurement of fermentation media.
In an attempt to resolve the difficult problem of on-line determination of the viscosity of non-Newtonian fermentation media, the authors have used a vibrating rod sensor mounted on a bioreactor. The sensor signal decreases nonlinearly with increased ...
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Saturday, June 28th, 2008
Enzymatic function of alginate immobilized rat hepatocytes.
The use of immobilized hepatocytes represents a promising approach for the problem of detoxification in acute hepatic failure. Hepatocyte viability and detoxification function of a number of complex enzyme systems were examined before and after immobilization in alginate droplets. ...
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Saturday, June 28th, 2008
Modeling dynamic experiments on the anaerobic degradation of molasses wastewater.
The kinetics of anaerobic degradation of a molasses wastewater were measured under constant pH conditions in a laboratory scale packed bed reactor. In continuous and batch experiments the formation and degradation rates of the organic acids ...
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Saturday, June 28th, 2008
Microheterogeneity of enzymes and deactivation.
The influence of microheterogeneity on enzyme inactivation kinetics is presented. Examples of different enzymes are given where microheterogeneity has been detected by different techniques. The different statistical models are presented which include the influence of microheterogeneity on enzyme inactivation kinetics and ...
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Saturday, June 28th, 2008
Recovery of penicillin by reactive extraction in centrifugal extractors.
Penicillin V and/or G were extracted from fermentation broth by Amberlite LA-2 in n-butylacetate at pH 5 in a laboratory centrifugal extractor, Type SA 01 of Westfalia, up to an overall phase throughput rate of 30 L/h, ...
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Saturday, June 28th, 2008
Formation of oligosaccharides during enzymatic lactose hydrolysis and their importance in a whey hydrolysis process: Part II: Experimental.
Enzymatic lactose hydrolysis using two yeast and two fungal lactases that are of current technical interest was studied. The enzymes were compared regarding their oligosaccharide production. Parameters influencing ...
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