Translational termination re-initiation between ORF1 and ORF2 enables capsid expression in a bovine
June 28th, 2008 | by admin |Translational termination re-initiation between ORF1 and ORF2 enables capsid expression in a bovine norovirus without the need for production of viral sub-genomic RNA.
A generally accepted view of norovirus replication is that capsid expression requires production of a sub-genomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polII-based baculovirus delivery system, we observed capsid expression following introduction of a full length genotype 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/re-initiation event between the non-structural and capsid ORFs, a feature that may be unique to genogroup 3 noroviruses.
McCormick CJ, Salim O, Lambden PR, Clarke IN.
Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom.