The novel gene AngRem104 downregulates glucocorticoid receptor expression and activates NF-kappaB in
March 16th, 2008 | by admin |The novel gene AngRem104 downregulates glucocorticoid receptor expression and activates NF-kappaB in human mesangial cells.
AngRem104 [angiotensin II (Ang II)-related genes in human mesangial cells (MCs), clone104], a novel gene in human MCs induced by Ang II, was previously identified in human MCs and found to interact with several proteins. The current study used a yeast two-hybrid system and co-immunoprecipitation to investigate the interaction between AngRem104 and glucocorticoid receptor (GR) AF-1-specific elongation factor (GR-EF). GR expression was downregulated and the number of MCs positive for activated nuclear factor kappaB (NF-kappaB) was increased when AngRem104 was overexpressed. Transfection with antisense AngRem104 vector resulted in the upregulation of GR protein and reduced numbers of MCs with activated NF-kappaB. These results indicate that the novel gene AngRem104 is involved in the in vivo regulation of GR expression and the activation of NF-kappaB through interaction with GR-EF in human MCs.
Zhang Y, Zhang H, Hou P, Liang X, Li Z, Wang H.
Renal Division, Peking University First Hospital, Institute of Nephrology, Peking University, No. 8, Xishiku Street, Beijing 100034, China; Department of Nephrology, Third Hospital, Hebei Medical University, Shijiazhuang 050051, China.