The novel 5bp deletion polymorphism in the promoter region of bovine ACRP30 gene.
May 1st, 2008 | by admin |The novel 5bp deletion polymorphism in the promoter region of bovine ACRP30 gene.
ACRP30 gene was located nearby the QTL affecting the marbling, ribeye muscle area and fat thickness on the BAT1 in Angus. In this study, a 5bp deletion mutation within the bovine ACRP30 gene was firstly detected and confirmed in 991 cattle by PCR-SSCP, DNA sequencing and direct PCR amplification. The deletion mutation was appeared in Qinchuan, Nanyang, Jiaxian and Hasake, but was not found in Jinnan, Chinese Holsteins and Angus. The association of the deletion polymorphism with growth traits (including birth weight, body weight, average daily gain and body sizes in different growth periods (6/12/18/24 month-old)) was analyzed in 224 Nanyang cattle. No signification association of the deletion polymorphism with growth traits were observed (P > 0.05). The deletion was located in the promoter region and it resulted in a new putative CCAAT/enhancer binding protein-beta response element (C/EBP-RE).
Zhang L, Chen H, Lan X, Zhang C, Zhang L, Zhang A, Zhang Q, Lei C, Zhang H.
College of Animal Science and technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, No. 22 Xinong Road, Yangling, Shaanx, 712100, P.R. China.