Spectroelectrochemistry of P700 in native photosystem I particles and diethyl ether-treated thylakoi
March 16th, 2008 | by admin |Spectroelectrochemistry of P700 in native photosystem I particles and diethyl ether-treated thylakoid membranes from spinach and Thermosynechococcus elongatus.
The redox potentials (E( composite function\’)) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3mV. Stepwise removal of antenna pigments by ether treatment caused distinct shifts of the E( composite function\’) value with increasing degree of water saturation in ether; negatively from +471 to +428mV for spinach, but positively from +423 to +436mV for T. elongatus. Such a contrasting behavior is discussed by invoking the mode of action of ether on the microenvironments around P700.
Zhang Y, Nakamura A, Kuroiwa Y, Kato Y, Watanabe T.
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.