[Specific T-cell responses to Mycobacterium tuberculosis protein ESAT-6 in Chinese HIV positive indi
June 27th, 2008 | by admin |[Specific T-cell responses to Mycobacterium tuberculosis protein ESAT-6 in Chinese HIV positive individuals.]
OBJECTIVE: To detect TB specific T cell responses by using the recombinant ESAT-6 protein as stimulus in Chinese HIV infected patients. METHODS: ELISPOT-IFN-gamma assay by using the recombinant ESAT-6 protein as stimulus to detect specific T cell responses in HIV(+) patients with or without clinical manifestation of TB diseases. RESULTS: Recombinant ESAT-6 protein specific T cell responses show significant high frequencies in both of TB patients with or without HIV infection than that in the healthy control and HIV(+) group without clinical TB diseases. CONCLUSION: The ELISPOT-IFN-gamma assay by using recombinant ESAT-6 protein as stimulus could be used in diagnoses of TB infection in Chinese HIV infected patients. [Key words] HIV Infection; Mycobo-cterium tuberculosis; Immunoenzyme techniques.
Zhang B, Lun WH, Cheng J, Zhao LF, Li XW, Han N, Wang Q, Liang YL, Mao Y.
The First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China. Corresponding author: LUN Wen-hui, E-mail: lunwenhui@163.com; Mao Yu, E-mail: mao58@vip.sohu.net.