Preparation, characterization and evaluation of marsupsin-phospholipid complex.
May 1st, 2008 | by admin |Preparation, characterization and evaluation of marsupsin-phospholipid complex.
The aim of this research was to formulate Marsupsin-phospholipid complex (M-P Complex) in attempt to increase the bioavailability of marsupsin and to characterize this new formulation along with its evaluation. Marsupsin-phospholipid complex was formulated by mechanical dispersion method. In this new formulation, complex formation was confirmed by carrying out transmission electron microscopy (TEM), IR, (1)H-NMR and RP-HPLC analysis. TEM showed M-P Complex diameter range of 0.05-0.5 mum. The entrapment efficiency of M-P Complex was found to be 44%. In vitro release study revealed its first order release profile. Mean blood serum concentration vs time curve of marsupsin was of first order after oral administration of M-P Complex in albino rabbits which clearly showed remarkably increased bioavailability of M-P Complex than standardized marsupsin. The average value of C (max) and T (max) of M-P Complex were found to be 3.02 mg/ml and 10.2 h, respectively. Hence the findings demonstrate that complexing marsupsin with phospholipids results in better oral bioavailability and improved biological response than free form of standardized marsupsin.
Sikarwar MS, Sharma S, Jain AK, Parial SD.
Department of Pharmacognosy and Phytochemistry, B. R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur, 458001, India, mukeshsikarwar@gmail.com.