[Pigment epithelium-derived factor expression in the retina in response to hypobaric hypoxia]
March 16th, 2008 | by admin |[Pigment epithelium-derived factor expression in the retina in response to hypobaric hypoxia]
OBJECTIVE: The present study was undertaken to examine the expression of pigment epithelium-derived factor (PEDF) in the retina of adult rat following hypobaric hypoxic exposures. METHODS: Forty-eight adult Wistar rats were divided into 6 groups randomly (8 rats in each group), of which 5 groups (40 rats) were subjected to hypobaric hypoxia for 2 hours following which expression of PEDF in the retina was investigated from 3 hours to 14 days by real time RT-PCR, western blotting and immunohistochemistry. RESULTS: PEDF mRNA and protein expression in the retina was decreased at 3 hours to 14 days following exposure to hypoxia. Compared with normal control group (1.00+/-0.00), PEDF mRNA expression decreased to 0.83+/-0.05 at 3 hours, 0.72+/-0.04 at 24 hours, 0.56+/-0.04 at 3 days, 0.54+/-0.05 at 7 days and 0.49+/-0.05 at 14 days respectively; Compared with normal control group (1.90+/-0.02), PEDF protein expression decreased to 1.49+/-0.02 at 3 hours, 1.19+/-0.05 at 24 hours, 1.15+/-0.05 at 3 days, 1.09+/-0.06 at 7 days and 1.07+/-0.03 at 14 days respectively. CONCLUSIONS: The findings of this study indicate hypoxia can downregulate PEDF in adult retina.
Zhang Y, Yuan YS, Xu L, Yuan LL.
Department of Ophthalmology, the First Hospital Affiliated to Kunming Medical College, Kunming 650031, China.