Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonanc
June 27th, 2008 | by admin |Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonance.
On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.
Tawa K, Hori H, Kintaka K, Kiyosue K, Tatsu Y, Nishii J.
Research Institute for Cell Engineering, AIST, Ikeda, Osaka 563-8577, Japan. tawa-keiko@aist.go.jp