NATURALLY ACQUIRED IgM ANTIBODY RESPONSE TO THE C-TERMINAL REGION OF THE MEROZOITE SURFACE PROTEIN 1
June 27th, 2008 | by admin |NATURALLY ACQUIRED IgM ANTIBODY RESPONSE TO THE C-TERMINAL REGION OF THE MEROZOITE SURFACE PROTEIN 1 OF PLASMODIUM VIVAX IN KOREA: USE FOR SERODIAGNOSIS OF VIVAX MALARIA.
The antibody levels against the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax (PvMSP1c) were measured in 276 patients with P. vivax malaria (patient group), 320 malaria-naïve healthy individuals (control group 1) and 70 malaria-naïve individuals with various disorders (control group 2), using the immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and the direct sandwich ELISA. To evaluate the antibody response during relapse, five relapsed patients were tested using the IgM capture ELISA. The IgM antibodies were negative in 99.7% of control group 1, 100% of control group 2 and were positive in 90.6% of the patient group, with the IgM capture ELISA. The total antibody levels were positive in 88.4% of the patient group with the direct sandwich ELISA. The sera from the second malaria episode, in relapsed patients, were 100% positive on the IgM capture ELISA. The results of this study suggest that the IgM capture ELISA may be a useful diagnostic method for P. vivax malaria for both primary infection and relapse.
Yeom JS, Kim ES, Lim KJ, Oh JH, Sohn MJ, Yoo SB, Kim E, Bae I, Jung YJ, Park JW.