[Microarray-based methods to identify DNA methylation in cancer.]
March 16th, 2008 | by admin |[Microarray-based methods to identify DNA methylation in cancer.]
DNA methylation is an epigenetic modification associated with gene transcription regulation, X-chromosome inactivation, regulation of development and cell differentiation. Aberrant DNA methylation has been linked to cancer. The advent of microarray technology has provided new opportunities for high-throughput study on DNA methylation. Various microarray-based methods depend on different pretreatments including immunoprecipitation and restriction digestion. Al-though each technique has its limitations, the immunoprecipitation method has high specificity, and the restriction digestion method shows high sensitivity. Together they provide many choices to the study of genome-wide DNA methylation profile in cancer.
Tian XQ, Sun DF, Zhang YJ, Fang JY.
Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China E-mail: txqwl2004@yahoo.com.cn.