Localization and Quantitative Analysis of Antigen-Antibody Binding on 2D Substrate Using Imaging Nan
June 27th, 2008 | by admin |Localization and Quantitative Analysis of Antigen-Antibody Binding on 2D Substrate Using Imaging NanoSIMS.
Iron containing-antigen bound specifically to antibody immobilized on a surface is analyzed by nanoscale secondary ion mass spectrometry (NanoSIMS). This technique is well adapted compared with X-ray photoelectron spectroscopy and energy dispersive spectroscopy, which do not allow the detection of iron. The obtained Fe (+) map gives a good representation of the antigen repartition on the surface. NanoSIMS analysis of competition experiments performed with albumin and iron-free antigen are in good accordance with results obtained by a classical fluorescence microscopy approach. These results underline the interest of imaging NanoSIMS as a label-free method, allowing the localization and quantitative analysis of antigen-antibody binding with better spatial resolution than imaging ellipsometry and SPR.
Dauphas S, Delhaye T, Lavastre O, Corlu A, Guguen-Guillouzo C, Ababou-Girard S, Geneste F.
Florence.Geneste@univ-rennes1.fr.