Lipase production byPenicillium restrictum in a bench-scale fermenter : Effect of carbon and nitroge
June 27th, 2008 | by admin |Lipase production byPenicillium restrictum in a bench-scale fermenter : Effect of carbon and nitrogen nutrition, agitation, and aeration.
A preliminary screening work selectedPenicillium restrictum as a promising micro-organism for lipase production. The physiological response of the fungus towards cell growth and enzyme production upon variable carbon and nitrogen nutrition, specific air flow rate (Qa) and agitation (N) was evaluated in a 5-L bench-scale fermenter. In optimized conditions for lipase production meat peptone at 2% (w/v) and olive oil at 1% (w/v) were used in a growth medium with a C/N ratio of 9.9. Higher C/N ratios favored cell growth in detriment of enzyme production. Low extracellular lipase activities were observed using glucose as carbon source suggesting glucose regulation. Final lipase accumulation of 13,000 U/L was obtained, using optimized specific air flow rate (Qa) of 0.5 wm and an impeller speed (N) of 200 rpm. Agitation showed to be an important parameter to ensure nutrient availability in a growth medium having olive oil as carbon source.
Freire DM, Teles EM, Bon EP, Anna GL.
Instituto de QuÃmica, Faculdade de Farmacia and COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.