Isoflavones and gamma irradiation inhibit cell growth in human salivary gland cells.
July 3rd, 2008 | by admin |Isoflavones and gamma irradiation inhibit cell growth in human salivary gland cells.
We studied the effects of isoflavones and irradiation on cell cycle in a human salivary gland cell line (HSG). Genistein and a soy isoflavone conjugate (NS) inhibited DNA synthesis. Cells deconjugated the glucoside form of isoflavones in NS to the aglycones genistein and daidzein. NS, genistein and IR increased phosphorylation of p53 and p21(CIP1) at serine 15 (phos-p53). Irradiation and NS also increased levels of p21(CIP1). In a cologenic survival assay, cells in log phase growth had high radio-sensitivity with 2Gy causing a reduction in survival (SF(2)=0.45). Conclusion: isoflavones and radiation may interact to sensitize cancer cells to radiation.
Katz J, Blake E, Medrano TA, Sun Y, Shiverick KT.
Department of Oral Maxillofacial Surgery and Diagnostic Sciences, University of Florida, P.O. Box 100414, Gainesville, FL 32610-0414, USA.