In Vitro Assembly of [Fe(4)S (4)] Cluster in High Potential Iron-Sulfur Protein from Acidithiobacill
June 27th, 2008 | by admin |In Vitro Assembly of [Fe(4)S (4)] Cluster in High Potential Iron-Sulfur Protein from Acidithiobacillus ferrooxidans.
High-potential iron-sulfur protein (HiPIP) has been proposed to be involved in the iron respiratory electron transport chain in Acidithiobacillus ferrooxidans, which contains an [Fe(4)S(4)] cluster. We report here the assembly of an [Fe(4)S(4)] cluster in HiPIP from A. ferrooxidans ATCC 23270 in vitro in the presence of Fe(2+) and sulfide. The spectra and matrix-assisted laser desorption ionization-time of flight mass spectrometry results of holoHiPIP confirmed that the iron-sulfur cluster was correctly assembled into the protein.
Zeng J, Jiang H, Geng M, Wang Y, Zhang X, Liu J, Qiu G.
Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, Changsha, Hunan, 410083, PRC, zengjcsu@yahoo.com.cn.