High Expression of l-Selectin Ligand in Secretory Endometrium is Associated with Better Endometrial
June 25th, 2008 | by admin |High Expression of l-Selectin Ligand in Secretory Endometrium is Associated with Better Endometrial Receptivity and Facilitates Embryo Implantation in Human Being.
Problem l-selectin ligand has displayed mediating adhesion at the maternal-fetal interface. Therefore, we investigated the impact of l-selectin ligand on establishing pregnancy in women undergoing in vitro fertilization and embryo transfer (IVF-ET). Method of study Endometrium between cycle days LH +6 to +9 was obtained from 56 Chinese women referred for IVF and tested for l-selectin ligand by immunohistochemistry and Western blot. The standard gonadotropin-releasing hormone agonist long protocol was used for ovarian stimulation. Results l-selectin ligand was localized in the endometrial gland and luminal epithelial cells. Western blot analysis of endometrium identified four bands and levels of component 1, 2 and 4 were significantly higher in the pregnancy group than in the non-pregnancy group (P < 0.05). Clinical pregnancy and implantation rates were higher in patients with high level l-selectin ligand compared with those with low level (53.6%versus 25.0%, and 27.1%versus 12.1%, respectively, P < 0.05). Conclusion The presence of higher level l-selectin ligand was associated with a better pregnancy outcome.
Wang B, Sheng JZ, He RH, Qian YL, Jin F, Huang HF.
Department of Reproductive Endocrinology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.