HUMORAL ANTIBODY RESPONSE OF THE TILAPIA OREOCHROMIS NILOTICUS AGAINST CICHLIDOGYRUS SPP. (MONOGENEA
June 27th, 2008 | by admin |HUMORAL ANTIBODY RESPONSE OF THE TILAPIA OREOCHROMIS NILOTICUS AGAINST CICHLIDOGYRUS SPP. (MONOGENEA).
The humoral immune response of the tilapia Oreochromis niloticus was evaluated using a direct ELISA. Serum was tested from fishes infected with Cichlidogyrus spp. (Monogenea), and from fishes injected intraperitoneally with the Cichlidogyrus spp. antigenic extract, i.e., 150 microl of the Cichlidogyrus spp. saline extract diluted in Freund s complete adjuvant (1:1) were inoculated intraperitoneally at day 0, followed by 2 dosages of 50 microl of the same Cichlidogyrus spp. saline extract diluted in Freund s incomplete adjuvant (1:1) at wk 2 and 4, respectively. The humoral response was also evaluated by the Double Immunodiffusion test (DID) and by serum protein and total immunoglobulin (Ig\’s) determinations. The IgM OD values in the hyper-immune fishes were significantly higher than in the infected and uninfected fish groups. In the DID test, a precipitation (antigen-antibody) band was observed between the Cichlidogyrus spp. saline extract and hyper-immune sera, but not with the other groups. Increases in serum protein concentration and total Ig\’s were observed at wk 2 and 10 post-injection in the immunized fish. Results from this study suggest that tilapia is capable of producing an induced humoral immune response against an antigenic extract of Cichlidogyrus spp.
Sandoval-GÃo J, RodrÃguez-Canul R, Vidal-Martinez V.