[Genotype 1 JEV was isolated again from Liaoning Province, China, 2007.]
June 27th, 2008 | by admin |[Genotype 1 JEV was isolated again from Liaoning Province, China, 2007.]
OBJECTIVE: To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene. METHODS: Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods. RESULTS: Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002. CONCLUSION: Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province. [Key words] Encephalitis virus, Japanese; Genotype; Phylogeny.
Meng WS, Li MH, Wang HY, Zhang JB, Gao Y, Ding J, Fu SH, Zhai YG, Chen Z, Gou JQ, Zhao YJ, Liang GD.
Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 100052, China. Corresponding author: LIANG Guo-dong E-mail: gdliang@hotmail.com.