[Genome analysis of a newly isolated enterovirus.]
June 27th, 2008 | by admin |[Genome analysis of a newly isolated enterovirus.]
OBJECTIVE: To demonstrate molecular characterization of a newly isolated enterovirus. METHODS: Virus were isolated from patient with unknown-causing disease and tested by reverse transcription-polymerase chain reaction (RT-PCR) and 5\’3\’RACE (rapid amplification of cDNA ends, RACE), in an attempt to obtain the sequence of this newly isolated enterovirus. RESULTS: Sequence analysis showed that this newly isolated enterovirus shared 83%-94% nucleotide identity and 91%-100% amino acid identity with enterovirus 89. Phylogenetic analysis indicated that it was probably a new subtype of enterovirus 89. CONCLUSION: This newly isolated enterovirus in the stool specimen from patient hasthe same serotype with enterovirus 89, but it was probably a new subtype of enterovirus 89. [Key words] Enterovirus; Genome; Genes,structural, viral.
Hou J, Hu Y, Sheng HH, Bai BK, Wang ZJ, Mao PY.
Department of Virology, Institute of Infectious Diseases, No. 302 Hospital, Beijing 100039, China. Corresponding author: MAO Pan-yong, E-mail: maopy302@yahoo.com.cn.