Function and transcriptional regulation of the isocitrate lyase in Pseudomonas aeruginosa.
June 27th, 2008 | by admin |Function and transcriptional regulation of the isocitrate lyase in Pseudomonas aeruginosa.
Pseudomonas aeruginosa ATCC 17933 is capable of growing aerobically on ethanol as sole source of carbon and energy. This requires the glyoxylate cycle for replenishing C(4)-compounds to the TCA cycle. The enzyme isocitrate lyase (ICL) catalyzes the first step of this glyoxylate shunt. Its activity was induced more than 10-fold in response to the carbon sources ethanol or acetate instead of glucose or succinate. We could prove that in P. aeruginosa ICL is essential for aerobic as well as anaerobic utilization of C(2)-sources. Transcriptional regulation of icl gene (aceA) expression was monitored on different carbon sources by using an aceA-lacZ gene fusion. A strong correlation between promoter and ICL activity indicated regulation at the transcriptional level. But ICL was not simply induced by the mere presence of ethanol in the growth medium as was demonstrated by cultivation on mixed substrates. P. aeruginosa showed diauxic growth on media containing ethanol-succinate or ethanol-glucose mixtures and did not transcribe the aceA gene to metabolize ethanol until succinate or glucose, respectively, were exhausted. Inactivation of the chromosomal aceA gene in P. aeruginosa led to an inability to grow on ethanol and acetate. Promoter activity studies showed that all genes necessary to oxidize ethanol were downregulated in the ICL-negative mutant. But on mixed substrates like ethanol-succinate or ethanol-glucose the mutant exhibited growth and utilized ethanol as well, probably as energy source only.
Kretzschmar U, Khodaverdi V, Jeoung JH, Görisch H.
Fachgebiet Technische Biochemie, Institut für Biotechnologie, Technische Universität Berlin, Seestr. 13, 13353, Berlin, Germany.