Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818).
June 28th, 2008 | by admin |Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818).
The single cell gel eletrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5, 5, 10 and 20Gy of (60)Co gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens.
Grazeffe VS, Tallarico LD, Pinheiro AD, Kawano T, Suzuki MF, Okazaki K, Pereira CA, Nakano E.
Laboratório de Parasitologia/Malacologia, Instituto Butantan, Avenida Vital Brasil, 1500, CEP 05503-900, São Paulo, Brazil.