Medical and Biomedical Researches

Dynamics of Poly(l-lysine) in Hyaluronic Acid/Poly(l-lysine) Multilayer Films Studied by Fluorescenc

Dynamics of Poly(l-lysine) in Hyaluronic Acid/Poly(l-lysine) Multilayer Films Studied by Fluorescence Recovery after Pattern Photobleaching.

Poly( l-lysine) (PLL)/hyaluronic acid (HA) multilayers are films whose thickness increases exponentially with the number of deposition steps. Such a growth process was attributed to the diffusion, in and out of the whole film, of at least one of the polyelectrolytes constituting the film. In the case of PLL/HA, PLL is known to be the diffusing species. In order to better understand the growth mechanism of such films, it is of primary importance to well characterize the diffusion process of the polyelectrolytes in the multilayer. This process is studied here by fluorescence recovery after pattern photobleaching. We show that the diffusion behavior is different when we consider either PLL chains that are deposited on top of the film or PLL chains embedded in the film, even below only one HA layer. For chains that are embedded, we find two populations: a mobile one with a diffusion coefficient, D, of the order of 0.1 microm (2).s (-1) and a population that appears immobile ( D < 0.001 microm (2).s (-1)). For chains deposited on top of the multilayer, a third population appears which is rapidly diffusing ( D congruent with 1 microm (2).s (-1)). These results confirm the validity of the model generally accepted for the exponential growth process and in particular the existence of up to three subgroups of PLL chains from the point of view of their diffusion coefficient.

Jourdainne L, Lecuyer S, Arntz Y, Picart C, Schaaf P, Senger B, Voegel JC, Lavalle P, Charitat T.

Institut National de la Santé et de la Recherche Médicale, Unité 595, 11 rue Humann, 67085 Strasbourg Cedex, France, Université Louis Pasteur, Faculté de Chirurgie Dentaire, 1 place de l’Hôpital, 67000 Strasbourg, France, Université Louis Pasteur, Institut Charles Sadron, CNRS UPR 22, 23 rue du loess, BP 84047, 67034 Strasbourg Cedex 2, France, Université de Montpellier II, Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Centre National de la Recherche Scientifique, UMR 5235, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France, and Hôpitaux Universitaires de Strasbourg, 1, Place de l’Hôpital, BP 426, 67091 Strasbourg Cedex, France philippe.lavalle@medecine.u-strasbg.fr.